The epidermis coordinates thermoresponsive growth through the phyB-PIF4-auxin pathway
| Autor: | Jungmook Kim, Jaewook Kim, Sara Kim, Geonhee Hwang, Hanim Kim, Jinkil Jeong, Soohwan Kim, Giltsu Choi, Thom Nguyen Thi, Eunkyoo Oh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Hot Temperature Science Arabidopsis General Physics and Astronomy Flowers 01 natural sciences Article General Biochemistry Genetics and Molecular Biology Plant Epidermis Hypocotyl 03 medical and health sciences Gene Expression Regulation Plant Phytochrome B Auxin Transcription (biology) Plant development Basic Helix-Loop-Helix Transcription Factors lcsh:Science Transcription factor Gene chemistry.chemical_classification Regulation of gene expression Multidisciplinary Indoleacetic Acids integumentary system biology Arabidopsis Proteins fungi food and beverages General Chemistry Plants Genetically Modified biology.organism_classification Vascular bundle Cell biology 030104 developmental biology chemistry lcsh:Q Plant sciences Signal Transduction 010606 plant biology & botany |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | In plants, an elevation in ambient temperature induces adaptive morphological changes including elongated hypocotyls, which is predominantly regulated by a bHLH transcription factor, PIF4. Although PIF4 is expressed in all aerial tissues including the epidermis, mesophyll, and vascular bundle, its tissue-specific functions in thermomorphogenesis are not known. Here, we show that epidermis-specific expression of PIF4 induces constitutive long hypocotyls, while vasculature-specific expression of PIF4 has no effect on hypocotyl growth. RNA-Seq and qRT-PCR analyses reveal that auxin-responsive genes and growth-related genes are highly activated by epidermal, but not by vascular, PIF4. Additionally, inactivation of epidermal PIF4 or auxin signaling, and overexpression of epidermal phyB suppresses thermoresponsive growth, indicating that epidermal PIF4-auxin pathways are essential for the temperature responses. Further, we show that high temperatures increase both epidermal PIF4 transcription and the epidermal PIF4 DNA-binding ability. Taken together, our study demonstrates that the epidermis regulates thermoresponsive growth through the phyB-PIF4-auxin pathway. The PIF4 transcription factor along with the phyB photoreceptor, regulates growth responses to elevated temperature in plants. Here the authors show that PIF4 expression in the epidermis, rather than the vasculature, stimulates auxin responses and thermoresponsive growth in Arabidopsis. |
| Databáze: | OpenAIRE |
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