LOTUS overexpression accelerates neuronal plasticity after focal brain ischemia in mice
| Autor: | Hajime Takase, Takaakira Yokoyama, Nobutaka Kawahara, Yuji Kurihara, Kohtaro Takei |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Central Nervous System Male lcsh:Medicine Vascular Medicine Nervous System Brain Ischemia Brain ischemia Mice 0302 clinical medicine Nerve Fibers Animal Cells Ischemia Nogo Receptor 1 Medicine and Health Sciences Medicine lcsh:Science Neurons Cerebral Ischemia Multidisciplinary Neuronal Plasticity Brain food and beverages Infarction Middle Cerebral Artery Stroke medicine.anatomical_structure Neurology Spinal Cord Medulla oblongata Brainstem medicine.symptom Anatomy Cellular Types Research Article Cerebrovascular Diseases Nogo Proteins Central nervous system Immunoblotting Mice Transgenic Brain damage 03 medical and health sciences Neuroplasticity Animals Ischemic Stroke business.industry Calcium-Binding Proteins fungi lcsh:R Biology and Life Sciences Cervical Cord Cell Biology medicine.disease Spinal cord Axons Mice Inbred C57BL Neuroanatomy Disease Models Animal 030104 developmental biology Cellular Neuroscience Brain Injuries lcsh:Q business Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | PLoS ONE, Vol 12, Iss 9, p e0184258 (2017) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Nogo receptor-1 (NgR1) and its ligands inhibit neuronal plasticity and limit functional recovery after brain damage such as ischemic stroke. We have previously shown that lateral olfactory tract usher substance (LOTUS) antagonizes NgR1-mediated signaling. Here, we investigated whether LOTUS enhances neuronal plasticity and functional recovery after brain focal ischemia in adult mice. Focal ischemic infarcts were induced in wild-type and LOTUS-overexpressing transgenic mice via middle cerebral artery occlusion. Endogenous LOTUS expression was increased in brain and cervical spinal cord of the contralateral side of ischemia in the chronic phase after brain ischemia. LOTUS overexpression accelerated midline-crossing axonal sprouting from the contralateral side to the ipsilateral side of ischemia in the medullar reticular formation and gray matter of denervated cervical spinal cord. Importantly, LOTUS overexpression improved neurological score highly correlated with laterality ratio of corticoreticular fibers of the medulla oblongata, indicating that LOTUS overexpression may overcome the inhibitory environment induced by NgR1 signaling for damaged motor pathway reconstruction after ischemic stroke. Thus, our data suggest that LOTUS overexpression accelerates neuronal plasticity in the brainstem and cervical spinal cord after stroke and LOTUS administration is useful for future therapeutic strategies. |
| Databáze: | OpenAIRE |
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