Role of fractalkine/CX3CR1 signaling pathway in the recovery of neurological function after early ischemic stroke in a rat model
Autor: | Chun Wang, Qian Wang, Geng-Sheng Mao, Yu-Song Ge, Yongzhong Lin, Dong-Mei Li, Yan-Zhi Liu |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Male Chemokine medicine.medical_specialty Blotting Western Interleukin-1beta CX3C Chemokine Receptor 1 Hippocampal formation General Biochemistry Genetics and Molecular Biology Brain Ischemia Brain ischemia Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Internal medicine CX3CR1 Medicine Animals RNA Messenger General Pharmacology Toxicology and Pharmaceutics CX3CL1 biology business.industry Chemokine CX3CL1 Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha Infarction Middle Cerebral Artery General Medicine medicine.disease Rats Blot Stroke Disease Models Animal 030104 developmental biology Endocrinology Immunology biology.protein Tumor necrosis factor alpha Receptors Chemokine Signal transduction business 030217 neurology & neurosurgery Signal Transduction |
Zdroj: | Life sciences. 184 |
ISSN: | 1879-0631 |
Popis: | This study aims to explore the role of fractalkine/CX3C chemokine receptor 1 (CX3CR1) signaling pathway in the recovery of neurological functioning after an early ischemic stroke in rats. After establishment of permanent middle cerebral artery occlusion (pMCAO) models, 50 rats were divided into blank, sham, model, positive control and CX3CR1 inhibitor groups. Neurological impairment, walking and grip abilities, and cortical and hippocampal infarctions were evaluated by Zea Longa scoring criterion, beam-walking assay and grip strength test, and diffusion-weighted magnetic resonance imaging. qRT-PCR and Western blotting were performed to detect mRNA and protein expressions. ELISA was conducted to measure concentration of sFractalkine (sFkn), interleukin-1β (IL-1β) and TNF-α. The recovery rate of neurological functioning impairment and reduced walking and grip abilities was faster in the positive control and CX3CR1 inhibitor groups than the model group. The model, positive control and CX3CR1 inhibitor groups showed increased mRNA and protein expression of chemokine C-X3-C motif ligand 1 (CX3CL1) and CX3CR1, concentration of sFkn, IL-1β and TNF-α, and size of cortical and cerebral infarctions while decreased expression of NGF and BDNF compared with the blank and sham groups. Compared with the model group, the mRNA and protein expression of CX3CL1 and CX3CR1, concentration of sFkn, IL-1β and TNF-α, and size of cortical and cerebral infarctions decreased while expression of NGF and BDNF increased in the positive control and CX3CR1 inhibitor groups. Thus, the study suggests that inhibition of fractalkine/CX3CR1 signaling pathway promotes the recovery of neurological functioning after the occurrence of an early ischemic stroke. |
Databáze: | OpenAIRE |
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