Role of fractalkine/CX3CR1 signaling pathway in the recovery of neurological function after early ischemic stroke in a rat model

Autor: Chun Wang, Qian Wang, Geng-Sheng Mao, Yu-Song Ge, Yongzhong Lin, Dong-Mei Li, Yan-Zhi Liu
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
Chemokine
medicine.medical_specialty
Blotting
Western
Interleukin-1beta
CX3C Chemokine Receptor 1
Hippocampal formation
General Biochemistry
Genetics and Molecular Biology
Brain Ischemia
Brain ischemia
Rats
Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Internal medicine
CX3CR1
Medicine
Animals
RNA
Messenger
General Pharmacology
Toxicology and Pharmaceutics
CX3CL1
biology
business.industry
Chemokine CX3CL1
Reverse Transcriptase Polymerase Chain Reaction
Tumor Necrosis Factor-alpha
Infarction
Middle Cerebral Artery
General Medicine
medicine.disease
Rats
Blot
Stroke
Disease Models
Animal
030104 developmental biology
Endocrinology
Immunology
biology.protein
Tumor necrosis factor alpha
Receptors
Chemokine
Signal transduction
business
030217 neurology & neurosurgery
Signal Transduction
Zdroj: Life sciences. 184
ISSN: 1879-0631
Popis: This study aims to explore the role of fractalkine/CX3C chemokine receptor 1 (CX3CR1) signaling pathway in the recovery of neurological functioning after an early ischemic stroke in rats. After establishment of permanent middle cerebral artery occlusion (pMCAO) models, 50 rats were divided into blank, sham, model, positive control and CX3CR1 inhibitor groups. Neurological impairment, walking and grip abilities, and cortical and hippocampal infarctions were evaluated by Zea Longa scoring criterion, beam-walking assay and grip strength test, and diffusion-weighted magnetic resonance imaging. qRT-PCR and Western blotting were performed to detect mRNA and protein expressions. ELISA was conducted to measure concentration of sFractalkine (sFkn), interleukin-1β (IL-1β) and TNF-α. The recovery rate of neurological functioning impairment and reduced walking and grip abilities was faster in the positive control and CX3CR1 inhibitor groups than the model group. The model, positive control and CX3CR1 inhibitor groups showed increased mRNA and protein expression of chemokine C-X3-C motif ligand 1 (CX3CL1) and CX3CR1, concentration of sFkn, IL-1β and TNF-α, and size of cortical and cerebral infarctions while decreased expression of NGF and BDNF compared with the blank and sham groups. Compared with the model group, the mRNA and protein expression of CX3CL1 and CX3CR1, concentration of sFkn, IL-1β and TNF-α, and size of cortical and cerebral infarctions decreased while expression of NGF and BDNF increased in the positive control and CX3CR1 inhibitor groups. Thus, the study suggests that inhibition of fractalkine/CX3CR1 signaling pathway promotes the recovery of neurological functioning after the occurrence of an early ischemic stroke.
Databáze: OpenAIRE
Externí odkaz: