Delta-Notch signaling is involved in the segregation of the three germ layers in Xenopus laevis
Autor: | Alejandra R. Paganelli, Andrés E. Carrasco, Diego Raul Revinski, Silvia L. López |
---|---|
Rok vydání: | 2009 |
Předmět: |
Mesoderm
Notch animal structures Otras Ciencias Biológicas Notch signaling pathway Ectoderm Germ layer Biology Xenopus Proteins Ciencias Biológicas purl.org/becyt/ford/1 [https] Xenopus laevis Xenopus Laevis Endopeptidases medicine Animals Neural ectoderm Neural Ectoderm purl.org/becyt/ford/1.6 [https] Molecular Biology Genetics Receptors Notch Gastrulation Endoderm Diploblasty Intracellular Signaling Peptides and Proteins Membrane Proteins Cell Biology Cell biology medicine.anatomical_structure Epiblast Delta embryonic structures Triploblasty Carrier Proteins CIENCIAS NATURALES Y EXACTAS Germ Layers Developmental Biology Signal Transduction |
Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
ISSN: | 1095-564X |
Popis: | In vertebrates, the induction of the three germ layers (ectoderm, mesoderm and endoderm) has been extensively studied, but less is known about how they segregate. Here, we investigated whether Delta-Notch signaling is involved in this process. Activating the pathway in the marginal zone with NotchICD resulted in an expansion of endodermal and neural ectoderm precursors, leaving a thinner mesodermal ring around the blastopore at gastrula stage, when germ layers are segregated. On the other hand, when the pathway was blocked with Delta-1STU or with an antisense morpholino oligonucleotide against Notch, the pan-mesodermal brachyury (bra) domain was expanded and the neural border was moved animalwards. Strikingly, the suprablastoporal endoderm was either expanded when Delta-1 signaling was blocked, or reduced after the general knock-down of Notch. In addition, either activating or blocking the pathway delays the blastopore closure. We conclude that the process of delimiting the three germ layers requires Notch signaling, which may be finely regulated by ligands and/or involve non-canonical components of the pathway. Moreover, Notch activity must be modulated at appropriate levels during this process in order to keep normal morphogenetic movements during gastrulation. Fil: Revinski, Diego Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina Fil: Paganelli, Alejandra Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina Fil: Carrasco, Andres Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina Fil: Lopez, Silvia Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina |
Databáze: | OpenAIRE |
Externí odkaz: |
Pro tento záznam nejsou dostupné žádné jednotky.