Promoter Methylation of RASSF1A Gene in Egyptian Patients with Ovarian Cancer
| Autor: | Mervat Harira, Amr Abd Almohsen Alnemr, Rasha H. Mohamed, Noha A. Rezk |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult endocrine system Tumor suppressor gene Bioengineering Biology Applied Microbiology and Biotechnology Biochemistry 03 medical and health sciences Ovarian tumor 0302 clinical medicine Promoter methylation medicine Humans Stage (cooking) Promoter Regions Genetic Molecular Biology Gene Ovarian Neoplasms Tumor Suppressor Proteins General Medicine Methylation DNA Neoplasm DNA Methylation Middle Aged medicine.disease 030104 developmental biology 030220 oncology & carcinogenesis DNA methylation Cancer research Egypt Female Ovarian cancer Biotechnology |
| Zdroj: | Applied biochemistry and biotechnology. 185(1) |
| ISSN: | 1559-0291 |
| Popis: | Ovarian malignancy is diagnosed in nearly a fourth of a million women internationally every year. Methylation of RASSF1A tumor suppressor gene prompts its inactivation in diseases. In this study, the RASSF1A promoter methylation was detected by methylated-specific PCR and investigated serum RASSF1A protein level through enzyme-linked immunosorbant assay in 160 Egyptian patients with ovarian cancer and 160 healthy controls. The present work proved that there was a higher frequency of RASSF1A methylation and a decrease in its serum level in patients with ovarian cancer compared to controls as well as in the high-grade tumor patients compared to low grade ones and also in advanced ovarian tumor stage compared to early stages. Our study exhibited that RASSF1A promoter hypermethylation and its protein levels may be a reliable and sensitive tool for diagnosing and monitoring of ovarian malignancy patients. |
| Databáze: | OpenAIRE |
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