In vivo imaging of intersynaptic vesicle exchange using VGLUT1(Venus) knock-in mice
| Autor: | U. Valentin Nägerl, Salah El Mestikawy, Fabien Nadrigny, Heinz Steffens, Christoph Biesemann, Katlin Silm, Jeong-Seop Rhee, Imke Helling, Etienne Herzog, Frank Kirchhoff, Richard Schwartzmann, Nils Brose, Tiphaine Bersot |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
0303 health sciences
Synaptic scaling Synaptic pharmacology General Neuroscience Neural facilitation Articles Biology Synaptic vesicle Cell biology 03 medical and health sciences 0302 clinical medicine Synaptic fatigue Synaptic augmentation Synaptic plasticity Glutamatergic synapse Neuroscience 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | Journal of Neuroscience Journal of Neuroscience; Vol 31 |
| Popis: | The vesicular glutamate transporter VGLUT1 loads synaptic vesicles with the neurotransmitter glutamate and thereby determines glutamate release at many synapses in the mammalian brain. Due to its function and selective localization, VGLUT1 is one of the most specific markers for glutamatergic synaptic vesicles. It has been used widely to identify glutamatergic synapses, and its expression levels are tightly correlated with changes in quantal size, modulations of synaptic plasticity, and corresponding behaviors. We generated a fluorescent VGLUT1Venusknock-in mouse for the analysis of VGLUT1 and glutamatergic synaptic vesicle trafficking. The mutation does not affect glutamatergic synapse function, and thus the new mouse model represents a universal tool for the analysis of glutamatergic transmitter systems in the forebrain. Previous studies demonstrated synaptic vesicle exchange between terminalsin vitro. Using the VGLUT1Venusknock-in, we show that synaptic vesicles are dynamically shared among boutons in the cortex of micein vivo. We provide a detailed analysis of synaptic vesicle sharingin vitro, and show that network homeostasis leads to dynamic scaling of synaptic VGLUT1 levels. |
| Databáze: | OpenAIRE |
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