Enhanced Hemostatic Performance of Tranexamic Acid-Loaded Chitosan/Alginate Composite Microparticles
| Autor: | Jiatao Zang, Pengxi Li, Donghong Li, Jiancang Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
Spectrophotometry Infrared Health Toxicology and Mutagenesis Composite number lcsh:Medicine Cell morphology Hemostatics Chitosan chemistry.chemical_compound Mice Glucuronic Acid Hemostatic Agent Cell Death Hexuronic Acids General Medicine Microspheres Tranexamic Acid Molecular Medicine Female Rabbits Powders Tranexamic acid Biotechnology medicine.drug Research Article medicine.medical_specialty Article Subject Alginates lcsh:Biotechnology Simulated body fluid Hemorrhage Absorption lcsh:TP248.13-248.65 Genetics medicine Animals MTT assay Particle Size Molecular Biology Cell Shape lcsh:R Water Fibroblasts Surgery Disease Models Animal chemistry Hemostasis Microscopy Electron Scanning Biomedical engineering |
| Zdroj: | Journal of Biomedicine and Biotechnology Journal of Biomedicine and Biotechnology, Vol 2012 (2012) |
| ISSN: | 1110-7251 1110-7243 |
| Popis: | Novel microparticles based on chitosan and sodium alginate were prepared using emulsification and cross-linking technologies. The spherical microparticles had a porous surface and a diameter of2~40 μm. In simulated body fluid, these microparticles quickly swelled but gradually degraded. The results of the MTT assay revealed that a slight inhibition of cell proliferation was observed on day 2 and then gradually decreased afterward. No cell morphology changes were observed. By loading tranexamic acid, the hemostatic performance of the microparticles was obviously improved. Using fast-acting styptic powder (Flashclot) as the control, the hemostatic efficiency was investigated in rabbits using a liver transection bleeding model. It was found that both Flashclot and the microparticles achieved hemostasis in3.07±0.84 min and2.48±0.88 min, respectively; however, the tranexamic acid-loaded microparticles stopped the bleeding in1.90±0.75 min (P<0.05). Additionally, Flashclot resulted in heat injury to the experimental livers, while the microparticles did not. Thus, with their biodegradability, safety, and superior hemostatic efficiency, tranexamic acid-loaded microparticles might be a promising new powdered hemostatic agent with a wide range of potential applications. |
| Databáze: | OpenAIRE |
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