Rapid PrPScDetection in Lymphoid Tissue and Application to Scrapie Surveillance of Fallen Stock in Japan: Variable PrPScAccumulation in Palatal Tonsil in Natural Scrapie
| Autor: | Takashi Yokoyama, Hiroko Hayashi, Kimi Shimada, Masuhiro Takata, Yoshifumi Iwamaru, Morikazu Imamura, Morikazu Shinagawa, Yuka Ookubo, Mary Jo Schmerr |
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| Rok vydání: | 2005 |
| Předmět: |
Pathology
medicine.medical_specialty PrPSc Proteins Lymphoid Tissue animal diseases Palatine Tonsil Immunology Scrapie Biology Microbiology Japan stomatognathic system Western blot Virology medicine Animals Genetic Predisposition to Disease Prion protein Sheep medicine.diagnostic_test nervous system diseases Lymphatic system medicine.anatomical_structure nervous system Tonsil |
| Zdroj: | Microbiology and Immunology. 49:801-804 |
| ISSN: | 0385-5600 |
| DOI: | 10.1111/j.1348-0421.2005.tb03660.x |
| Popis: | Rapid western blot (WB) procedure for an abnormal isoform of prion protein (PrP(Sc) ) detection in lymphoid tissues was established and has been applied to the surveillance of fallen stock. In this program, brain and palatal tonsil were examined by WB and three cases of sheep scrapie were detected. While one clinically scrapie-infected sheep harbored PrP(Sc) in the brain and palatal tonsil, the two sheep in the pre-clinical stage harbored PrP(Sc) in the brain, but not in the palatal tonsil. This study shows that PrP(Sc) accumulation in palatal tonsil is variable in natural scrapie, even among genetically susceptible sheep. |
| Databáze: | OpenAIRE |
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