Combinatorial action of Grainyhead, Extradenticle and Notch in regulating Hox mediated apoptosis in Drosophila larval CNS
| Autor: | Risha Khandelwal, Sriivatsan G. Rajan, Raviranjan Kumar, Rohit Joshi, Rashmi Sipani |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Central Nervous System Male Cancer Research Apoptosis Nervous System Biochemistry Database and Informatics Methods Binding Analysis 0302 clinical medicine RNA interference Cell Signaling Medicine and Health Sciences Drosophila Proteins Hox gene Genetics (clinical) Genetics Regulation of gene expression Notch Signaling Cell Death Receptors Notch Genes Homeobox Gene Expression Regulation Developmental Nuclear Proteins Nucleic acids Enhancer Elements Genetic Genetic interference Cell Processes Larva Epigenetics Drosophila Female Anatomy Sequence Analysis Research Article Signal Transduction animal structures lcsh:QH426-470 Bioinformatics Notch signaling pathway Biology Research and Analysis Methods 03 medical and health sciences Neuroblast Sequence Motif Analysis DNA-binding proteins Animals Amino Acid Sequence Enhancer Molecular Biology Techniques Transcription factor Molecular Biology Ecology Evolution Behavior and Systematics Chemical Characterization Homeodomain Proteins fungi Biology and Life Sciences Proteins Cell Biology lcsh:Genetics 030104 developmental biology Homeobox RNA Gene expression 030217 neurology & neurosurgery Cloning Transcription Factors |
| Zdroj: | PLoS Genetics PLoS Genetics, Vol 13, Iss 10, p e1007043 (2017) |
| ISSN: | 1553-7404 1553-7390 |
| Popis: | Hox mediated neuroblast apoptosis is a prevalent way to pattern larval central nervous system (CNS) by different Hox genes, but the mechanism of this apoptosis is not understood. Our studies with Abdominal-A (Abd-A) mediated larval neuroblast (pNB) apoptosis suggests that AbdA, its cofactor Extradenticle (Exd), a helix-loop-helix transcription factor Grainyhead (Grh), and Notch signaling transcriptionally contribute to expression of RHG family of apoptotic genes. We find that Grh, AbdA, and Exd function together at multiple motifs on the apoptotic enhancer. In vivo mutagenesis of these motifs suggest that they are important for the maintenance of the activity of the enhancer rather than its initiation. We also find that Exd function is independent of its known partner homothorax in this apoptosis. We extend some of our findings to Deformed expressing region of sub-esophageal ganglia where pNBs undergo a similar Hox dependent apoptosis. We propose a mechanism where common players like Exd-Grh-Notch work with different Hox genes through region specific enhancers to pattern respective segments of larval central nervous system. Author summary Specification of the head to tail axis of the developing central nervous system is carried out by Hox genes. Hox mediated programmed cell death of the neural progenitor cells plays an important role in specification of this axis, but the molecular mechanism of this phenomenon is not well understood. We have studied this phenomenon in abdominal and subesophageal regions of larval central nervous system of Drosophila. We find that different Hox genes use a combination of common players (Extradenticle, Grainyhead and Notch) but employ region specific enhancers to cause progenitor cell death in different segments of developing central nervous system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|