CRISPR/Cas9-Mediated Deletion of Foxn1 in NOD/SCID/IL2rg−/− Mice Results in Severe Immunodeficiency
| Autor: | Baiheng Li, Peng Li, Lin Simiao, Suna Wang, Le Qin, Liangxue Lai, Youdi Xu, Yao Yao, Qiting Wu, Pentao Liu, Qiuhua Deng, Xinru Wei, Donghai Wu, Guohua Huang, Yunxin Lai, Qiubin Liang |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Severe combined immunodeficiency Multidisciplinary business.industry T cell Science Gene targeting Nod medicine.disease 3. Good health Hairless Transplantation 03 medical and health sciences Leukemia 030104 developmental biology medicine.anatomical_structure Immunology Cancer research Medicine business Immunodeficiency |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-12 (2017) |
| ISSN: | 2045-2322 |
| Popis: | Immunodeficient mice engrafted with either normal or cancerous human cells are widely used in basic and translational research. In particular, NOD/SCID/IL2rg−/− mice can support the growth of various types of human cancer cells. However, the hairs of these mice interfere with the observation and imaging of engrafted tissues. Therefore, novel hairless strains exhibiting comparable immunodeficiency would be beneficial. Recently, the CRISPR/Cas9 system has been used for efficient multiplexed genome editing. In the present study, we generated a novel strain of nude NOD/SCID/IL2rg−/− (NSIN) mice by knocking out Foxn1 from NOD/SCID/IL2rg−/− (NSI) mice using the CRISPR/Cas9 system. The NSIN mice were deficient in B, T, and NK cells and not only showed impaired T cell reconstitution and thymus regeneration after allogeneic bone marrow nucleated cell transplantation but also exhibited improved capacity to graft both leukemic and solid tumor cells compared with NSI, NOG, and NDG mice. Moreover, the NSIN mice facilitated the monitoring and in vivo imaging of both leukemia and solid tumors. Therefore, our NSIN mice provide a new platform for xenograft mouse models in basic and translational research. |
| Databáze: | OpenAIRE |
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