Reciprocal Self‐Assembly of Peptide–DNA Conjugates into a Programmable Sub‐10‐nm Supramolecular Deoxyribonucleoprotein
| Autor: | Mahnseok Kye, Yong Beom Lim |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Deoxyribonucleoprotein Base pair Deoxyribonucleoproteins Supramolecular chemistry Nanotechnology 02 engineering and technology Conjugated system 010402 general chemistry 01 natural sciences DNA Antisense Catalysis 03 medical and health sciences chemistry.chemical_compound Humans Chemistry General Medicine DNA General Chemistry 021001 nanoscience & nanotechnology Nanostructures 0104 chemical sciences 030104 developmental biology Covalent bond Thermodynamics Protein Conformation beta-Strand Self-assembly Peptides 0210 nano-technology HeLa Cells Conjugate |
| Zdroj: | Angewandte Chemie International Edition. 55:12003-12007 |
| ISSN: | 1521-3773 1433-7851 |
| DOI: | 10.1002/anie.201605696 |
| Popis: | To overcome the limitations of molecular assemblies, the development of novel supramolecular building blocks and self-assembly modes is essential to create more sophisticated, complex, and controllable aggregates. The self-assembly of peptide-DNA conjugates (PDCs), in which two orthogonal self-assembly modes, that is, β-sheet formation and Watson-Crick base pairing, are covalently combined in one supramolecular system, is reported. Despite extensive research, most self-assembly studies have focused on using only one type of building block, which restricts structural and functional diversity compared to multicomponent systems. Multicomponent systems, however, suffer from poor control of self-assembly behaviors. Covalently conjugated PDC building blocks are shown to assemble into well-defined and controllable nanostructures. This controllability likely results from the decrease in entropy associated with the restriction of the molecular degrees of freedom by the covalent constraints. Using this strategy, the possibility to thermodynamically program nano-assemblies to exert gene regulation activity with low cytotoxicity is demonstrated. |
| Databáze: | OpenAIRE |
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