Effects of diethyldithiocarbamate and nickel chloride on glutathione and trace metal concentrations in rat liver
| Autor: | James F. Belliveau, G.P. O'Leary, M.C. Reid, O. Zaharia, F.W. Sunderman, H. Griffin |
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| Rok vydání: | 1984 |
| Předmět: |
Male
medicine.medical_specialty chemistry.chemical_element Zinc Toxicology Chloride chemistry.chemical_compound Nickel Thiocarbamates Internal medicine Mole medicine Animals Metallothionein Sodium diethyldithiocarbamate Radioisotopes Manganese integumentary system fungi Drug Synergism Metabolism Glutathione Rats Inbred F344 Rats Drug Combinations Endocrinology Liver Biochemistry chemistry Food Ditiocarb Copper medicine.drug |
| Zdroj: | Toxicology. 32:11-21 |
| ISSN: | 0300-483X |
| Popis: | Concentrations of reduced glutathione (GSH) and oxidized glutathione (GSSG) and 4 trace metals (Ni, Cu, Mn, Zn) were measured in livers from rats treated with sodium diethyldithiocarbamate (DDC, 0.67 or 1.33 mmol/ kg, i.m.) and NiCl 2 (0.25 or 0.50 mmol/kg, s.c.), singly or in combination. In rats treated with DDC or NiCl 2 , singly, hepatic GSH was diminished at 4 h and returned to control levels (or slightly above) at 17 h. In rats that received DDC plus NiCl 2 , hepatic GSH was not diminished at 4 h and was increased 1.4–1.8-fold at 17 h. Hepatic GSSG was diminished at 4 h after NiCl 2 treatment and returned to control values at 17 h; hepatic GSSG did not differ from control values at 4 h or 17 h after treatment with DDC, alone or combined with NiCl 2 . Hepatic Ni was below the detection limit (∼ 20 nmol/g) in control and DDC-treated rats; hepatic Ni was increased to 53 ± 26 (S.D.) nmol/g at 17 h after treatment with NiCl 2 alone, and was increased 6-fold (308 ± 63 nmol/g in rats that received Ni plus DDC. Under the same conditions, hepatic Zn was increased 33% or 41%, respectively, in rats that received NiCl 2 or DDC, singly, and was not further increased by combined treatment; hepatic Cu and Mn concentrations were unaffected by NiCl 2 or DDC, singly, but were diminished in rats that received NiCl 2 and DDC. This study suggests: (a) that increased hepatic uptake of Ni is largely responsible for the synergistic induction of heme oxygenase activity in rats treated with NiCl 2 and DDC; and (b) that increased hepatic uptake of Zn contributes to the induction of hepatic metallothionein by NiCl 2 and DDC. |
| Databáze: | OpenAIRE |
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