Use of Wheat Straw for Value-Added Product Xylanase by Penicillium chrysogenum Strain A3 DSM105774
Autor: | Amira M. Embaby, A. I. Khalil, Amira A. Matrawy, H. S. Marey |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Microbiology (medical)
xylanase Strain (chemistry) biology wheat straw QH301-705.5 low cost eco-friendly methodology Plant Science Straw Penicillium chrysogenum biology.organism_classification Box–Behnken design Article chemistry.chemical_compound Penicillium chrysogenum strain A3 DSM105774 chemistry Xylanase Yeast extract Fermentation Ammonium Food science Biology (General) Ecology Evolution Behavior and Systematics submerged state of fermentation |
Zdroj: | Journal of Fungi Journal of Fungi, Vol 7, Iss 696, p 696 (2021) Volume 7 Issue 9 |
ISSN: | 2309-608X |
Popis: | The present work highlights the valorization of the bulky recalcitrant lignocellulose byproduct wheat straw (WS) for the enhanced production of value-added xylanase by the locally sourced novel Penicillium chrysogenum strain A3 DSM105774 for the first time. The optimized production of xylanase by submerged state of fermentation of WS was achieved using a three-step statistical and sequential approach: one factor at a time (OFAT), Plackett–Burman design (PBD), and Box Behnken design (BBD). Incubation temperature (30 °C), WS, and ammonium sulphate were the key determinants prompting xylanase production inferred from OFAT. The WS concentration (%(w/v)), yeast extract concentration (%(w/v)), and initial pH of the production medium imposed significant effects (p ≤ 0.05) on the produced xylanase, realized from PBD. The predicted levels of WS concentration, initial pH of the production medium, and yeast extract concentration provoking the ultimate xylanase levels (53.7 U/mL) with an 8.95-fold enhancement, localized by the estimated ridge of the steepest ascent of the ridge analysis path, were 3.8% (w/v), 5.1, and 0.098% (w/v), respectively 94.7% lab validation. The current data underpin the up-scaling of xylanase production using this eco-friendly, cheap, and robust methodology for the valorization of WS into the value-added product xylanase. |
Databáze: | OpenAIRE |
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