The novel outer membrane protein from OprD/Occ family is associated with hypervirulence of carbapenem resistant Acinetobacter baumannii ST2/KL22

Autor: Xinmin Xu, Huizhu Wang, Chen Chen, Mingxi Hua, Xinzhe Liu, Ang Li, Yajie Wang, Yu Jiang, Jingyuan Liu, Pengcheng Du, Hui Zeng, Min Li
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Microbiology (medical)
Acinetobacter baumannii
Male
animal structures
antibiotic resistance
Virulence Factors
Immunology
Microbial Sensitivity Tests
Infectious and parasitic diseases
RC109-216
Microbiology
acinetobacter baumanii
03 medical and health sciences
Antibiotic resistance
Drug Resistance
Multiple
Bacterial
polycyclic compounds
Animals
Phylogeny
030304 developmental biology
0303 health sciences
biology
Virulence
030306 microbiology
Critically ill
orpd
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
biology.organism_classification
kl22
Anti-Bacterial Agents
Mice
Inbred C57BL
sequence types 2
Infectious Diseases
Carbapenems
bacteria
Parasitology
Bacterial outer membrane
Carbapenem resistant Acinetobacter baumannii
Research Article
Research Paper
Bacterial Outer Membrane Proteins
Zdroj: Virulence, Vol 12, Iss 1, Pp 1-11 (2021)
Virulence
article-version (VoR) Version of Record
ISSN: 2150-5608
2150-5594
Popis: Acinetobacter baumannii has become a major healthcare threat that causes nosocomial infections, especially in critically ill patients. The spread of carbapenem-resistant A. baumannii (CRAB) strains has long been a clinical concern. It is important to study the epidemiology and virulence characteristics of different CRAB isolates in order to tailor infection prevention and antibiotic prescribing. In this study, a total of 71 CRAB isolates were collected in the hospital, and clinical characteristics of infections were analyzed. The genomic characteristics and phylogenetic relationships were elucidated based on genome sequencing and analysis. The isolates were assigned to three sequence types (STs, Pasteur) and nine capsular polysaccharide (KL) types, among which ST2/KL22 was the most prevalent CRAB in the hospital. Even though all the ST2/KL22 isolates contained the same reported virulence genes, one specific clade of ST2/KL22 showed more pathogenic in mouse infection model. Complete genomic analysis revealed differences at the oprD locus between the low- and high-virulent isolates. More specifically, a premature stop codon in the low-virulence strains resulted in truncated OprD expression. By evaluating pathogenicity in C57BL/6 J mice, knock-out of oprD in high-virulent isolate resulted in virulence attenuation, and complementing the avirulent strain with full-length oprD from high-virulent isolate enhanced virulence of the former. The oprD gene may be associated with the enhanced virulence of the specific ST2/KL22 clone, which provides a potential molecular marker for screening the hypervirulent A. baumannii strains.
Databáze: OpenAIRE
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