Freeze-drying of solutions of serum albumin containing dimethylsulfoxide
| Autor: | Basil T. Doumas, Theodore I. Malinin, Billy W. Perry, Donald Greiff |
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| Rok vydání: | 1976 |
| Předmět: |
Chromatography
Cryobiology Magnetic Resonance Spectroscopy biology Chemistry Oxide Serum albumin General Medicine Nuclear magnetic resonance spectroscopy General Biochemistry Genetics and Molecular Biology Freeze-drying chemistry.chemical_compound Freeze Drying Deuterium biology.protein Humans Sublimation (phase transition) Dimethyl Sulfoxide General Agricultural and Biological Sciences Serum Albumin |
| Zdroj: | Cryobiology. 13(2) |
| ISSN: | 0011-2240 |
| Popis: | Although several publications have emphasized the inadvisability of drying biologic materials containing dimethylsulfoxide (DMSO) by sublimation of ice in vacuo, the current studies showed a relationship to exist between the relative concentrations of serum albumin, human, and dimethylsulfoxide for successful or unsuccessful freeze-drying of albumin-DMSO solutions. The cycle of freeze-drying for the successful drying of an albumin-DMSO solution was a modification of the cycle used for the successful drying of suspensions of measles virus by sublimation of ice in vacuo. Using nuclear magnetic resonance spectroscopy, a strong, sharp signal for DMSO was obtained in preparations of freeze-dried albumin-DMSO solutions rehydrated with deuterium oxide, D2O. |
| Databáze: | OpenAIRE |
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