Protection by extra virgin olive oil against oxidative stress in vitro and in vivo. Chemical and biological studies on the health benefits due to a major component of the Mediterranean diet
Autor: | Fabio Gionfra, Stuart Belli, Jens Pedersen, Miriam Rossi, Lorraine Kwok, Nora Molasky, Elena Candelotti, Francesco Caruso, Sandra Incerpi, Daniela Tofani, Kathleen M. Raley-Susman, Stefano Leone, Tomáš Filipský, Alessio Caruso, Grace Lee |
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Přispěvatelé: | Rossi, M, Caruso, F, Kwok, L, Lee, G, Caruso, A, Gionfra, F, Candelotti, E, Belli, Sl, Molasky, N, Raley-Susman, Km, Filipský, T, Tofani, D, Pedersen, J, Incerpi, S |
Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
Animals
Caenorhabditis elegans Cell Cycle Cell Line Electron Spin Resonance Spectroscopy Humans In Vitro Techniques Olive Oil Oxidative Stress Paraquat Rats Reactive Oxygen Species Diet Mediterranean 0301 basic medicine Antioxidant medicine.medical_treatment lcsh:Medicine Mediterranean medicine.disease_cause Biochemistry Antioxidants Monocytes Myoblasts chemistry.chemical_compound White Blood Cells 0302 clinical medicine Galvinoxyl Superoxides Animal Cells Plant Products Medicine and Health Sciences Electrochemistry Food science lcsh:Science chemistry.chemical_classification Multidisciplinary Superoxide Stem Cells Agriculture Oxides Lipids Peroxides Hydroperoxide Chemistry 030220 oncology & carcinogenesis Physical Sciences Cellular Types Research Article Immune Cells Immunology Settore BIO/09 Vegetable Oils 03 medical and health sciences medicine Settore BIO/10 Reactive oxygen species Blood Cells Electrode Potentials lcsh:R Chemical Compounds Biology and Life Sciences Cell Biology Agronomy Diet Tyrosol 030104 developmental biology chemistry Hydroxytyrosol lcsh:Q Oils Oxidative stress Crop Science |
Zdroj: | PLoS ONE PLoS ONE, Vol 12, Iss 12, p e0189341 (2017) |
Popis: | We report the results of in vivo studies in Caenorhabditis elegans nematodes in which addition of extra virgin olive oil (EVOO) to their diet significantly increased their life span with respect to the control group. Furthermore, when nematodes were exposed to the pesticide paraquat, they started to die after two days, but after the addition of EVOO to their diet, both survival percentage and lifespans of paraquat-exposed nematodes increased. Since paraquat is associated with superoxide radical production, a test for scavenging this radical was performed using cyclovoltammetry and the EVOO efficiently scavenged the superoxide. Thus, a linear correlation (y = -0.0838x +19.73, regression factor = 0.99348) was observed for superoxide presence (y) in the voltaic cell as a function of aliquot (x) additions of EVOO, 10 μL each. The originally generated supoeroxide was approximately halved after 10 aliquots (100 μL total). The superoxide scavenging ability was analyzed, theoretically, using Density Functional Theory for tyrosol and hydroxytyrosol, two components of EVOO and was also confirmed experimentally for the galvinoxyl radical, using Electron Paramagnetic Resonance (EPR) spectroscopy. The galvinoxyl signal disappeared after adding 1 μL of EVOO to the EPR cell in 10 minutes. In addition, EVOO significantly decreased the proliferation of human leukemic THP-1 cells, while it kept the proliferation at about normal levels in rat L6 myoblasts, a non-tumoral skeletal muscle cell line. The protection due to EVOO was also assessed in L6 cells and THP-1 exposed to the radical generator cumene hydroperoxide, in which cell viability was reduced. Also in this case the oxidative stress was ameliorated by EVOO, in line with results obtained with tetrazolium dye reduction assays, cell cycle analysis and reactive oxygen species measurements. We ascribe these beneficial effects to EVOO antioxidant properties and our results are in agreement with a clear health benefit of EVOO use in the Mediterranean diet. |
Databáze: | OpenAIRE |
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