Second harmonic generation microscopy is a novel technique for differential diagnosis of breast fibroepithelial lesions
| Autor: | Wai Jin Tan, Hanry Yu, Boon-Huat Bay, Min-Han Tan, Jie Yan, Puay Hoon Tan, Shuoyu Xu, Aye Aye Thike |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty Biopsy Breast Neoplasms Pathology and Forensic Medicine Diagnosis Differential Phyllodes Tumor Microscopy medicine Humans Tissue microarray medicine.diagnostic_test biology business.industry General Medicine Second Harmonic Generation Microscopy medicine.disease Immunohistochemistry Primary and secondary antibodies Fibroadenoma Tissue Array Analysis biology.protein Female Collagen Differential diagnosis business |
| Zdroj: | Journal of Clinical Pathology. 68:1033-1035 |
| ISSN: | 1472-4146 0021-9746 |
| DOI: | 10.1136/jclinpath-2015-203231 |
| Popis: | Breast fibroepithelial lesions, including fibroadenomas and phyllodes tumours, are commonly encountered in clinical practice. As histological differences between these two related entities may be subtle, resulting in a challenging differential diagnosis, pathological techniques to assist the differential diagnosis of these two entities are of high interest. An accurate diagnosis at biopsy is important given corresponding implications for clinical decision-making including surgical extent and monitoring. Second harmonic generation (SHG) microscopy is a recently developed optical imaging technique capable of robust, powerful and unbiased label-free direct detection of collagen fibril structure in tissue without the use of antibodies. We constructed tissue microarrays emulating limited materials on biopsy to investigate quantitative collagen signal in fibroepithelial lesions using SHG microscopy. Archived formalin-fixed paraffin-embedded materials of 47 fibroepithelial lesions (14 fibroadenomas and 33 phyllodes tumours) were evaluated. Higher collagen signal on SHG microscopy was observed in fibroadenomas than phyllodes tumours on SHG imaging (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|