RIN4 recruits the exocyst subunit EXO70B1 to the plasma membrane
| Autor: | Peter Sabol, Viktor Žárský, Ivan Kulich |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Protein family Physiology animal diseases medicine.medical_treatment Protein subunit Arabidopsis Vesicular Transport Proteins Pseudomonas syringae chemical and pharmacologic phenomena Exocyst Plant Science Aquaporins 01 natural sciences Cell membrane 03 medical and health sciences Bacterial Proteins Autophagy medicine Secretion RIN4 EXO70B2 Protease Arabidopsis Proteins Effector Chemistry EXO70B1 Cell Membrane Intracellular Signaling Peptides and Proteins biochemical phenomena metabolism and nutrition Research Papers Cell biology secretion exocyst 030104 developmental biology medicine.anatomical_structure bacteria sense organs plant immunity Carrier Proteins 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/erx007 |
| Popis: | The exocyst subunit EXO70B1 is recruited to the plasma membrane by the immunity-related RIN4 protein and this change in localization is manipulated by a Pseudomonas syringae effector possibly to inhibit PAMP-triggered immunity. The exocyst is a conserved vesicle-tethering complex with principal roles in cell polarity and morphogenesis. Several studies point to its involvement in polarized secretion during microbial pathogen defense. In this context, we have found an interaction between the Arabidopsis EXO70B1 exocyst subunit, a protein which was previously associated with both the defense response and autophagy, and RPM1 INTERACTING PROTEIN 4 (RIN4), the best studied member of the NOI protein family and a known regulator of plant defense pathways. Interestingly, fragments of RIN4 mimicking the cleavage caused by the Pseudomonas syringae effector protease, AvrRpt2, fail to interact strongly with EXO70B1. We observed that transiently expressed RIN4, but not the plasma membrane (PM) protein aquaporin PIP2, recruits EXO70B1 to the PM. Unlike EXO70B1, RIN4 does not recruit the core exocyst subunit SEC6 to the PM under these conditions. Furthermore, the AvrRpt2 effector protease delivered by P. syringae is able to release both RIN4 and EXO70B1 to the cytoplasm. We present a model for how RIN4 might regulate the localization and putative function of EXO70B1 and speculate on the role the AvrRpt2 protease might have in the regulation of this defense response. |
| Databáze: | OpenAIRE |
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