Functional differences in mesenchymal stromal cells from human dental pulp and periodontal ligament
| Autor: | Ramesh R. Bhonde, Vulugundam Sowmya Jahnavi, Susarla Jyothi Prasanna, Anoop Babu Vasandan, Shilpa Rani Shankar, Priya Prasad |
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| Rok vydání: | 2014 |
| Předmět: |
immune properties
Stage-Specific Embryonic Antigens Stromal cell Periodontal Ligament Cellular differentiation T cell Gene Expression Priming (immunology) Biology pro-inflammatory cytokines Osteocytes Immunophenotyping Proinflammatory cytokine Interferon-gamma Chondrocytes stomatognathic system stem cells Antigens CD Adipocytes medicine Humans dental pulp stromal cells Cells Cultured Dental Pulp Cell Proliferation Tumor Necrosis Factor-alpha Mesenchymal stem cell periodontal ligament stromal cells Cell Differentiation Mesenchymal Stem Cells Original Articles differentiation HLA-DR Antigens Cell Biology Chondrogenesis Cell biology medicine.anatomical_structure Organ Specificity Immunology Molecular Medicine Stem cell mesenchymal stromal cells Biomarkers |
| Zdroj: | Journal of Cellular and Molecular Medicine |
| ISSN: | 1582-4934 1582-1838 |
| DOI: | 10.1111/jcmm.12192 |
| Popis: | Clinically reported reparative benefits of mesenchymal stromal cells (MSCs) are majorly attributed to strong immune-modulatory abilities not exactly shared by fibroblasts. However, MSCs remain heterogeneous populations, with unique tissue-specific subsets, and lack of clear-cut assays defining therapeutic stromal subsets adds further ambiguity to the field. In this context, in-depth evaluation of cellular characteristics of MSCs from proximal oro-facial tissues: dental pulp (DPSCs) and periodontal ligament (PDLSCs) from identical donors provides an opportunity to evaluate exclusive niche-specific influences on multipotency and immune-modulation. Exhaustive cell surface profiling of DPSCs and PDLSCs indicated key differences in expression of mesenchymal (CD105) and pluripotent/multipotent stem cell-associated cell surface antigens: SSEA4, CD117, CD123 and CD29. DPSCs and PDLSCs exhibited strong chondrogenic potential, but only DPSCs exhibited adipogenic and osteogenic propensities. PDLSCs expressed immuno-stimulatory/immune-adhesive ligands like HLA-DR and CD50, upon priming with IFNγ, unlike DPSCs, indicating differential response patterns to pro-inflammatory cytokines. Both DPSCs and PDLSCs were hypo-immunogenic and did not elicit robust allogeneic responses despite exposure to IFNγ or TNFα. Interestingly, only DPSCs attenuated mitogen-induced lympho-proliferative responses and priming with either IFNγ or TNFα enhanced immuno-modulation capacity. In contrast, primed or unprimed PDLSCs lacked the ability to suppress polyclonal T cell blast responses. This study indicates that stromal cells from even topographically related tissues do not necessarily share identical MSC properties and emphasizes the need for a thorough functional testing of MSCs from diverse sources with respect to multipotency, immune parameters and response to pro-inflammatory cytokines before translational usage. |
| Databáze: | OpenAIRE |
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