Effect of a Silver Nanoparticles Solution on Staphylococcus aureus and Candida spp

Autor: Camila Cristina de Foggi, Amanda Fucci Wady, Camila Andrade Zamperini, Eduardo Buozi Moffa, Valtencir Zucolotto, Ana Lucia Machado, Carlos Eduardo Vergani
Přispěvatelé: Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP)
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Zdroj: Journal of Nanomaterials, Vol 2014 (2014)
Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 1687-4129
1687-4110
Popis: Made available in DSpace on 2015-03-18T15:55:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-01-01Bitstream added on 2015-03-18T16:28:38Z : No. of bitstreams: 1 WOS000341999000001.epub: 2038650 bytes, checksum: eb2354a02a6b0b99edcc269db91678e9 (MD5)Bitstream added on 2015-03-18T16:28:38Z : No. of bitstreams: 2 WOS000341999000001.epub: 2038650 bytes, checksum: eb2354a02a6b0b99edcc269db91678e9 (MD5) WOS000341999000001.pdf: 2451485 bytes, checksum: 293716596554680daf525ab5a4d55108 (MD5) Made available in DSpace on 2015-03-18T15:55:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-01-01Bitstream added on 2015-03-18T16:28:38Z : No. of bitstreams: 1 WOS000341999000001.epub: 2038650 bytes, checksum: eb2354a02a6b0b99edcc269db91678e9 (MD5)Bitstream added on 2015-03-18T16:28:38Z : No. of bitstreams: 2 WOS000341999000001.epub: 2038650 bytes, checksum: eb2354a02a6b0b99edcc269db91678e9 (MD5) WOS000341999000001.pdf: 2451485 bytes, checksum: 293716596554680daf525ab5a4d55108 (MD5) Made available in DSpace on 2015-03-18T15:55:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-01-01Bitstream added on 2015-03-18T16:28:38Z : No. of bitstreams: 1 WOS000341999000001.epub: 2038650 bytes, checksum: eb2354a02a6b0b99edcc269db91678e9 (MD5)Bitstream added on 2015-03-18T16:28:38Z : No. of bitstreams: 2 WOS000341999000001.epub: 2038650 bytes, checksum: eb2354a02a6b0b99edcc269db91678e9 (MD5) WOS000341999000001.pdf: 2451485 bytes, checksum: 293716596554680daf525ab5a4d55108 (MD5) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação para o Desenvolvimento da UNESP (FUNDUNESP) An AgNPs solution was synthesized by chemical reduction, characterized, and tested against Candida glabrata, Candida tropicalis, Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus (MRSA). Minimum inhibitory (MICs) and minimum fungicidal/bactericidal concentrations (MFC/MBC) were determined on planktonic cells. Also, total biofilm mass was determined by crystal violet (CV) staining and morphological changes by scanning electron microscope (SEM). MICs for C. glabrata, C. tropicalis, S. aureus, and MRSA were 15.63, 3.91, 1.95, and 1.95 mu g/mL, respectively. MFC for C. glabrata was 62.5 mu g/mL and for C. tropicalis 15.63 mu g/mL The same MBC (3.91 mu g/mL) was observed for S. aureus and MRSA. CV assay showed that the AgNPs (1000 mu g/mL) promoted reductions in biofilm mass of similar to 60% for C. glabrata and similar to 35% for C. tropicalis. A reduction of similar to 20% in C. tropicalis biomass was also observed at the concentration of 3.91 mu g/mL. No significant effect on total biomass was found for S. aureus and MRSA. SEM images revealed that C. glabrata and C. tropicalis biofilm cells, exposed to the AgNPs (1000 mu g/mL), had an irregular and shriveled appearance. AgNPs solution exhibited considerable antimicrobial activity against important fungal and bacterial pathogens, associated with several oral and systemic diseases, and has potential as an antimicrobial agent. Univ Estadual Paulista, UNESP, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, BR-14801903 Araraquara, SP, Brazil Univ Sao Paulo, Phys Inst Sao Carlos, Nanomed & Nanotoxicol Grp, BR-13566590 Sao Carlos, SP, Brazil Univ Estadual Paulista, UNESP, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, BR-14801903 Araraquara, SP, Brazil FAPESP: 08/07454-9
Databáze: OpenAIRE
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