Analysis of Benzodiazepines in Dynamically Coated Capillaries by CE-DAD, CE-MS and CE-MS2
| Autor: | Denis De Keukeleire, Gerd Vanhoenacker, F De L'escaille, Pat Sandra |
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| Rok vydání: | 2004 |
| Předmět: |
Chromatography
Chemistry Clinical Biochemistry Extraction (chemistry) Analytical chemistry Electrophoresis Capillary Pharmaceutical Science Electrolyte Mass spectrometry Diode array Mass Spectrometry Analytical Chemistry Benzodiazepines Capillary electrophoresis Double coating Drug Discovery Electrolyte composition Urine sample Spectroscopy |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 34:595-606 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/s0731-7085(03)00571-5 |
| Popis: | The applicability of a low pH volatile electrolyte for fast analysis of benzodiazepines with CE-MS was investigated. The electrolyte is based on a commercially available CEofix buffer system that produces a substantial and highly reproducible electroosmotic flow through a dynamic double coating principle. The system was first evaluated with a mixture of benzodiazepine standards in CE-DAD and the electrolyte composition was further optimized for CE-MS. The LOD for the six selected benzodiazepines with CE-MS was ca. 100 ppb, except for diazepam, for which the LOD was lower than 50 ppb. RSDs varied from 0.51 to 1.02% (n=7) for migration times and from 4.75 to 11.80% (n=7) for peak areas. The method was successfully applied to the analysis of a spiked urine sample after solid-phase extraction (SPE). CE-MS2 was performed on a standard mixture. |
| Databáze: | OpenAIRE |
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