A regulatory code for neurogenic gene expression in theDrosophilaembryo
| Autor: | Angela Stathopoulos, Robert P. Zinzen, Peter Markstein, Michael Levine, Ka Ping Yee, Michele Markstein, Albert Erives |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Embryo Nonmammalian Molecular Sequence Data Enhancer RNAs Ectoderm Biology Nervous System Polymerase Chain Reaction Genome Mesoderm Anopheles Gene expression medicine Animals Drosophila Proteins Cloning Molecular Enhancer Molecular Biology Genetics Base Sequence Gene Expression Regulation Developmental Embryo Cell biology Repressor Proteins Gastrulation Drosophila melanogaster Enhancer Elements Genetic medicine.anatomical_structure Gene Expression Regulation Mutagenesis Sequence motif Transcription Factors Developmental Biology |
| Zdroj: | Development. 131:2387-2394 |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.01124 |
| Popis: | Bioinformatics methods have identified enhancers that mediate restricted expression in the Drosophila embryo. However, only a small fraction of the predicted enhancers actually work when tested in vivo. In the present study, co-regulated neurogenic enhancers that are activated by intermediate levels of the Dorsal regulatory gradient are shown to contain several shared sequence motifs. These motifs permitted the identification of new neurogenic enhancers with high precision: five out of seven predicted enhancers direct restricted expression within ventral regions of the neurogenic ectoderm. Mutations in some of the shared motifs disrupt enhancer function, and evidence is presented that the Twist and Su(H) regulatory proteins are essential for the specification of the ventral neurogenic ectoderm prior to gastrulation. The regulatory model of neurogenic gene expression defined in this study permitted the identification of a neurogenic enhancer in the distant Anopheles genome. We discuss the prospects for deciphering regulatory codes that link primary DNA sequence information with predicted patterns of gene expression. |
| Databáze: | OpenAIRE |
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