Mg, K-containing microparticle: A possible active principle of a culture extract produced by a microbial consortium
| Autor: | Haruhisa Kikuchi, Toru Higashinakagawa, Hidekazu Kuwayama |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Dictyosteliomycota
Potassium Social Sciences Microscopy Atomic Force Lactic Acid Bacteria chemistry.chemical_compound Spectrum Analysis Techniques Energy Dispersive Spectroscopy Sociology Filter Paper Consortia Magnesium Dictyostelium Chemical Neutralization Chromatography Reverse-Phase Multidisciplinary Dictyostelium Discoideum Chemical Reactions Eukaryota Protists Microbial consortium Lactic acid Laboratory Equipment Chemistry Experimental Organism Systems Slime Molds Physical Sciences Engineering and Technology Medicine Photosynthetic bacteria Research Article Chemical Elements Science Liquid-Liquid Extraction Microbial Consortia Equipment chemistry.chemical_element Research and Analysis Methods Model Organisms Column chromatography Lactic Acid Particle Size Microparticle Chromatography Bacteria Protozoan Models Fungi Organisms Aqueous two-phase system Biology and Life Sciences Yeast Culture Media chemistry Animal Studies Electron Beam Spectrum Analysis Techniques |
| Zdroj: | PLoS ONE, Vol 16, Iss 11 (2021) PLoS ONE, Vol 16, Iss 11, p e0259297 (2021) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | A synthetic microbial consortium called Effective Microorganisms (EM) consists mainly of photosynthetic bacteria, lactic acid bacteria and yeast. Various effects of EM∙XGOLD, a health drink produced by EM, on life cycle of Dictyostelium discoideum were described previously. Here, we report our attempt to identify the active principle, termed EMF, that brought about the observed effects. Throughout the purification processes, the presence of the active principle was monitored by promoted fruiting body formation. By liquid-liquid separation the activity was recovered in aqueous phase, which, after concentration, was further subjected to reverse-phase column chromatography. No activity was detected in any eluant, while almost all the activity was recovered in residual insoluble material. The application of conventional organic chemistry procedures to the residual fraction did not lead to any informative results. Acid treatment of the insoluble material produced air bubbles, suggesting it to be composed of some inorganic carbonate. Viewed under scanning electronmicroscope, the residue revealed spherical particles of μm size range. Energy Dispersive X-ray (EDX) Spectroscopy pointed to the existence, on the surface of the particles, of magnesium and, to a certain extent, of potassium. In separate experiments, acid treatment and alkali neutralization of EM∙XGOLD completely wiped out the stimulatory activity of fruiting body formation. These lines of evidence indicate these Mg, K-containing microparticles to be an active principle of EM culture extract. How these particles exert their effect is currently under intensive investigation. |
| Databáze: | OpenAIRE |
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