Phytochemical Constituents, ChEs and Urease Inhibitions, Antiproliferative and Antioxidant Properties of Elaeagnus umbellata Thunb
| Autor: | Ibrahim Demirtas, Tevfik Ozen, Muhammed Altun, Semiha Yenigun |
|---|---|
| Přispěvatelé: | Ondokuz Mayıs Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
antiproliferative activity
Hydroxybenzoic acid Antioxidant E. umbellata Thunb. (EU) medicine.medical_treatment Linoleic acid phenolics Flavonoid Phytochemicals Ethyl acetate antioxidant activity 01 natural sciences Antioxidants Rutin chemistry.chemical_compound Structure-Activity Relationship Drug Discovery medicine Animals Humans Food science Horses Enzyme Inhibitors Cell Proliferation chemistry.chemical_classification urease Elaeagnaceae Dose-Response Relationship Drug 010405 organic chemistry Organic Chemistry General Medicine acetylcholinesterase Ascorbic acid Antineoplastic Agents Phytogenic Urease 0104 chemical sciences Computer Science Applications High-Throughput Screening Assays 010404 medicinal & biomolecular chemistry Biochemistry chemistry Phytochemical butyrylcholinesterase Acetylcholinesterase Drug Screening Assays Antitumor HT29 Cells HeLa Cells |
| Popis: | Yenigun, Semiha/0000-0002-1979-5427 WOS: 000413456900010 PubMed: 28137214 Aim and Objective: Due to the common ethnopharmacological used or scientifically examined biochemical properties, Elaeagnaceae family, Elaeagnus umbellate (Thunb.) (EU, Guz yemisi) was worth investigating. Materials and Methods: In this investigation, we revealed antioxidant, antiproliferative and enzyme inhibition activities of the water, methanol, ethanol, acetone, ethyl acetate and hexane extracts of EU as well as the contents of their phenolic, flavonoid, anthocyanin, ascorbic acid, lycopene and beta-carotene. The antioxidant activity was screened by total antioxidant (phosphomolybdenum), inhibition of linoleic acid peroxidation, reducing power, 2-deoxyribose degradation assay, H2O2 scavenging and metal chelating activities of the samples were tested in vitro. Additionally, the scavenging activities of the extracts were determined against 1,1-diphenyl-2-picrylhydrazyl (DPPH.), 2,2-azino-bis(3-ethylbenzothiazloine-6-sulfonicacid (ABTS(center dot+)), superoxide anion and peroxide radicals. The samples were determined for their inhibitory activities against urease, acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). In vitro, antiproliferative activities of six different extracts were tested using the xCELLigence system against HeLa and HT29 cell lines. Results: The antioxidant activities of the extracts were found higher than standard antioxidants. The water extracts of fruit and leaf showed the best antioxidant activity. In inhibition assays of urease, AChE and BuChE, all extracts exhibited remarkable inhibition potential. Ethyl acetate extracts, especially, showed better inhibition capacity. It was found that the antioxidant activities of the extracts presented consistently with their chemical contents. The antiproliferative activities of leaf extracts were more effective than the fruit extracts. The chromatographic methods were applied to the different solvents to analyses phenolic secondery metabolites. It was found that fumaric acid, 4-hydroxybenzoic acid, rutin and quercetin-3-beta-D-glucoside, neohesperidin, hesperidin determined to have higher contents all the extracts. Conclusion: EU can be suggested as a potential natural source of antioxidants appropriate for utilization in nutritional/pharmaceutical fields. Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [114Z683] We would like to thank the Scientific and Technological Research Council of Turkey (TUBITAK) for financial support (114Z683). |
| Databáze: | OpenAIRE |
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