Fermented milk with Lactobacillus curvatus SMFM2016-NK alleviates periodontal and gut inflammation, and alters oral and gut microbiota
Autor: | Yohan Yoon, Eun Young Park, Yewon Lee, Young-Geun Choi, Hong Ghi Lee, Hyemin Oh, Joohyun Kang, Sujung Lee, Kyu-Sil Choi, Jimyeong Ha, Young Keun Kim, Yeongeun Seo, Sejeong Kim |
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Rok vydání: | 2021 |
Předmět: |
food.ingredient
Gut flora Microbiology Proinflammatory cytokine Rodent Diseases 03 medical and health sciences Cecum fluids and secretions food Lactobacillus rhamnosus Skimmed milk Genetics medicine Animals Microbiome Feces 030304 developmental biology Inflammation Periodontitis 0303 health sciences biology Probiotics 0402 animal and dairy science food and beverages 04 agricultural and veterinary sciences biology.organism_classification medicine.disease 040201 dairy & animal science Gastrointestinal Microbiome Rats Lactobacillus Milk medicine.anatomical_structure Animal Science and Zoology Food Science |
Zdroj: | Journal of Dairy Science. 104:5197-5207 |
ISSN: | 0022-0302 |
Popis: | This study aimed to analyze the effect of milk fermented with Lactobacillus curvatus SMFM2016-NK on periodontal diseases and gut health in a rat model. To improve the effect of Lb. curvatus SMFM2016-NK-fermented milk administration for relieving periodontitis, the periodontitis rat models were treated with the following for 4 wk: 10% skim milk (normal), periodontitis + 10% skim milk (negative control), periodontitis + Lactobacillus rhamnosus GG-fermented milk (positive control), and periodontitis + Lb. curvatus SMFM2016-NK-fermented milk (PD+LCFM). Transcriptional analysis of inflammatory cytokines [tumor necrosis factor α (TNF-α), IL-1β, IL-6, and IL-10] was performed via quantitative reverse-transcription PCR. The changes in the oral and gut microbiomes after administering Lb. curvatus SMFM2016-NK-fermented milk were analyzed with metagenomics sequencing using DNA extracted from the oral gingival tissues and feces from the cecum of the rat models. After treatment with Lb. curvatus SMFM2016-NK-fermented milk, the relative gene expression levels of TNFA and IL1B in the gingiva decreased in the PD+LCFM group compared with those in the negative control group. In the oral microbiome, the proportion of the phylum Proteobacteria in the PD+LCFM group was lower than that in the negative control after treatment with Lb. curvatus SMFM2016-NK-fermented milk. For the effect in the gut, the relative gene expression levels of inflammatory cytokines in the colon between the normal and negative control groups were not different; however, the expression levels of TNFA and IL1B in the PD+LCFM and positive control groups, respectively, were lower than those in the negative control group. The composition and diversity of the gut microbiome differed among normal, periodontitis, and Lb. curvatus SMFM2016-NK-fermented milk treatment groups. These results indicate that Lb. curvatus SMFM2016-NK-fermented milk could alleviate periodontal and gut inflammation and change oral and gut microbiota. |
Databáze: | OpenAIRE |
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