Modelling human CNS injury with human neural stem cells in 2- and 3-Dimensional cultures
| Autor: | Olivia Gillham, Patrizia Ferretti, Barbora Vagaska |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Central Nervous System
Cell Survival Central nervous system Cell Culture Techniques lcsh:Medicine Biology Neuroprotection Article Neuroblastoma In vivo Cell Line Tumor medicine Humans Trauma Nervous System lcsh:Science Cells Cultured Embryonic Stem Cells Biological models Cell Proliferation Neural stem cells Neurons Regulation of gene expression Multidisciplinary lcsh:R Gene Expression Regulation Developmental Cell Differentiation medicine.disease Phenotype Neural stem cell Oxygen Glucose medicine.anatomical_structure Cell culture Thapsigargin lcsh:Q Neuroscience |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-62906-y |
| Popis: | The adult human central nervous system (CNS) has very limited regenerative capability, and injury at the cellular and molecular level cannot be studied in vivo. Modelling neural damage in human systems is crucial to identifying species-specific responses to injury and potentially neurotoxic compounds leading to development of more effective neuroprotective agents. Hence we developed human neural stem cell (hNSC) 3-dimensional (3D) cultures and tested their potential for modelling neural insults, including hypoxic-ischaemic and Ca2+-dependent injury. Standard 3D conditions for rodent cells support neuroblastoma lines used as human CNS models, but not hNSCs, but in all cases changes in culture architecture alter gene expression. Importantly, response to damage differs in 2D and 3D cultures and this is not due to reduced drug accessibility. Together, this study highlights the impact of culture cytoarchitecture on hNSC phenotype and damage response, indicating that 3D models may be better predictors of in vivo response to damage and compound toxicity. |
| Databáze: | OpenAIRE |
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