Polyribosome and ribonucleoprotein complex redistribution of mRNA induced by GnRH involves both EIF2AK3 and MAPK signaling
| Autor: | Hiral Dave, Mark A. Lawson, Minh-Ha Do, Rachel E. Intriago, Feng He, Uriah A. Astorga, Tae-Shin Kim, Sonia Jain |
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| Rok vydání: | 2013 |
| Předmět: |
endocrine system
MAP Kinase Signaling System Gonadotropin-releasing hormone Biology Biochemistry Article Cell Line Gonadotropin-Releasing Hormone Mice eIF-2 Kinase Endocrinology Polysome Gene expression Protein biosynthesis Animals RNA Messenger Extracellular Signal-Regulated MAP Kinases Molecular Biology 3' Untranslated Regions Protein Kinase C Ribonucleoprotein Sirolimus Messenger RNA Endoplasmic reticulum Dual Specificity Phosphatase 1 Enzyme Activation ELAV Proteins Ribonucleoproteins Polyribosomes Protein Biosynthesis Unfolded protein response Unfolded Protein Response Tetradecanoylphorbol Acetate |
| Zdroj: | Molecular and cellular endocrinology. 382(1) |
| ISSN: | 1872-8057 |
| Popis: | The neuropeptide gonadotropin-releasing hormone stimulates synthesis and secretion of the glycoprotein gonadotropic hormones and activates the unfolded protein response, which causes a transient reduction of endoplasmic reticulum-associated mRNA translation. Hormone-treated cell extracts were fractionated to resolve mRNA in active polyribosomes from mRNA in inactive complexes. Quantitative real-time PCR and expression array analysis were used to determine hormone-induced redistribution of mRNAs between fractions and individual mRNAs were found to be redistributed differentially. Among the affected mRNAs relevant to gonadotropin synthesis, the luteinizing hormone subunit genes Lhb and Cga were enriched in the ribonucleoprotein pool. The MAP kinase phosphatase Dusp1 was enriched in the polyribosome pool. Enrichment of Dusp1 mRNA in the polyribosome pool was independent of the unfolded protein response, sensitive to ERK inhibition, and dependent on the 3′ untranslated region. The results show that GnRH exerts translational control to modulate physiologically relevant gene expression through two distinct signaling pathways. |
| Databáze: | OpenAIRE |
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