Effects of antibiotics on the growth and differentiation in dissociated brain cell cultures
| Autor: | F. Amonn, Ulrich N. Wiesmann, Norbert Herschkowitz, Karel Hofmann, U. Baumann |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
medicine.drug_class
Cellular differentiation Antibiotics Nerve Tissue Proteins Penicillins Biology Microbiology chemistry.chemical_compound Mice medicine Animals Cells Cultured Sulfoglycosphingolipids Cell growth General Neuroscience Brain Cell Differentiation DNA Antimicrobial Penicillin chemistry Animals Newborn Streptomycin Gentamicin Gentamicins medicine.drug |
| Zdroj: | Neuroscience. 3(4-5) |
| ISSN: | 0306-4522 |
| Popis: | Penicillin, streptomycin or gentamicin in medium concentrations routinely used in cell culture interfere with the levels of total protein and DNA and with the synthesis of sulfatide (a component of the myelin membrane and indicator of cell differentiation) in brain cell cultures of newborn mice. At 6, 9 and 12 days in culture, the combination of 200 I.U. penicillin and 50 μg streptomycin/ml as well as 50 μg streptomycin alone depresses total protein, DNA and sulfatide synthesis. Up to 300 I.U. penicillin or 50 μg gentamicin/ml shows no effect on protein and DNA while there is a dose-dependent interference with sulfatide synthesis. Less than 100 I.U. penicillin or 6 μg gentamicin/ml do not affect sulfatide synthesis. Since the antimicrobial activity is preserved even at lower concentrations, the doses of antibiotics routinely used in cell culture should be reduced to levels no longer affecting apparent cell metabolism. The cultures could, however, be used to study the effects of drugs other than antibiotics on cellular growth and differentiation. |
| Databáze: | OpenAIRE |
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