Bioglass incorporated methacrylated collagen bioactive ink for 3D printing of bone tissue
| Autor: | Thuy-Uyen Nguyen, Nilabh S. Kajave, Vipuil Kishore, Akhilesh K. Gaharwar, Trevor Schmitt |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Ceramics
0206 medical engineering Biomedical Engineering Bioengineering 02 engineering and technology Bone tissue Bone tissue engineering Bone and Bones Article Biomaterials Collagen network medicine Humans health care economics and organizations Body fluid Tissue Engineering Tissue Scaffolds Chemistry Mesenchymal stem cell Mesenchymal Stem Cells 021001 nanoscience & nanotechnology 020601 biomedical engineering humanities medicine.anatomical_structure Self-healing hydrogels Printing Three-Dimensional Alkaline phosphatase Ink Collagen Swelling medicine.symptom 0210 nano-technology Biomedical engineering |
| Zdroj: | Biomed Mater |
| ISSN: | 1748-605X |
| Popis: | Bioactive three-dimensional (3D) printed scaffolds are promising candidates for bone tissue engineering (BTE) applications. Here, we introduce a bioactive ink composed of Bioglass 45S5 (BG) and methacrylated collagen (CMA) for 3D printing of biomimetic constructs that resemble the organic and inorganic composition of native bone tissue. A uniform dispersion of BG particles within the collagen network improved stability and reduced swelling of collagen hydrogels. Rheological testing showed significant improvement in the yield stress and percent recovery of 3D printed constructs upon BG incorporation. Further, addition of BG improved the bone bioactivity of 3D printed constructs in stimulated body fluid. BG incorporated CMA (BG-CMA) constructs maintained high cell viability and enhanced alkaline phosphatase activity of human mesenchymal stem cells. In addition, cell-mediated calcium deposition was significantly higher on BG-CMA constructs, compared to CMA alone. In conclusion, 3D printed BG-CMA constructs have significant potential for use in BTE applications. |
| Databáze: | OpenAIRE |
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