Investigation on the morphological protective effect of 5-hydroxymethylfurfural extracted from wine-processed Fructus corni on human L02 hepatocytes
| Autor: | Lihong Ye, Li-Hua Jiang, Xu Zhang, Chenghua Lou, Feng-ming Zhao, Yu Li, Baochang Cai, Xia Ding, Mingyan Wang, Xiao-Yi Yu, Hai-Yan Peng, Guangming Yang, Dong-Qing Xu |
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| Rok vydání: | 2010 |
| Předmět: |
Apoptosis
Enzyme-Linked Immunosorbent Assay Wine Pharmacognosy Cell Line Cornus Microscopy Electron Transmission Drug Discovery Humans Medicine Furaldehyde Microscopy Phase-Contrast Mode of action Cell Shape Pharmacology biology Traditional medicine Caspase 3 Plant Extracts business.industry Hydrogen Peroxide Cornus officinalis biology.organism_classification Cytoprotection Caspase 9 In vitro medicine.anatomical_structure Microscopy Fluorescence Biochemistry Cell culture Hepatocyte Hepatocytes business |
| Zdroj: | Journal of Ethnopharmacology. 130:424-428 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/j.jep.2010.05.024 |
| Popis: | Aim: To determine the mode of action of 5-hydroxymethylfurfural (5-HMF) extracted from wineprocessed Fructus corni on hepatoprotective activities, the effects of 5-HMF on H2O2-induced human L02 hepatocytes injury was examined. Mthods: Hepatocytes L02 injured by H2O2 was treated by 5-HMF. The morphological changes of the cells were observed under inverted phase-contrast, fluorescence, and transmission electron microscopy and the activities of caspase-9 and caspase-3 were tested by enzyme-linked immunosorbent detector. Results: It revealed that 5-HMF improved the morphology of H2O2-treated human L02 hepatocytes, and also inhibited the level of caspase-9 and caspase-3 of them. Conclusions: These results suggested a morphological hepatocyte protective effect and the anti-apoptosis mechanism by 5-HMF. © 2010 Elsevier Ireland Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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