Discovery of a novel oocyte-specific Krüppel-associated box domain-containing zinc finger protein required for early embryogenesis in cattle
| Autor: | George W. Smith, Prasanthi P. Koganti, Kun Zhang, Kristen Mastrantoni, Jianbo Yao, Jacqelyn M. Hand, Lei Wang, Mohamed Ashry, Sandeep K. Rajput |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Embryology Small interfering RNA Embryonic Development Gene Expression Biology 03 medical and health sciences Krüppel Transcription (biology) Gene expression Transcriptional regulation Animals Humans Amino Acid Sequence Cloning Molecular Transcription factor Zinc finger Base Sequence Gene Expression Regulation Developmental Zinc Fingers Molecular biology Intracellular signal transduction Repressor Proteins Protein Transport 030104 developmental biology HEK293 Cells Oocytes Cattle Rabbits Developmental Biology |
| Zdroj: | Mechanisms of development. 144 |
| ISSN: | 1872-6356 |
| Popis: | Zinc finger (ZNF) transcription factors interact with DNA through zinc finger motifs and play important roles in a variety of cellular functions including cell growth, proliferation, development, apoptosis, and intracellular signal transduction. One-third of ZNF proteins in metazoans contain a highly conserved N-terminal motif known as the Kruppel-associated box (KRAB) domain, which acts as a potent, DNA-binding dependent transcriptional repression module. Analysis of RNA-Seq data generated from a bovine oocyte cDNA library identified a novel transcript, which encodes a KRAB-containing ZNF transcription factor (named ZNFO). Characterization of ZNFO mRNA expression revealed that it is exclusively expressed in bovine oocytes and early embryos. A GFP reporter assay demonstrated that ZNFO protein localizes specifically to the nucleus, supporting its role in transcriptional regulation. To test the role of ZNFO in early embryonic development, zygotes were generated by in vitro maturation and fertilization of oocytes, and injected with small interfering RNA (siRNA) designed to knockdown ZNFO. Cleavage rates were not affected by ZNFO siRNA injection. However, embryonic development to 8- to 16-cell stage and blastocyst stage was significantly reduced relative to the uninjected and negative control siRNA-injected embryos. Further, interaction of ZNFO with the highly conserved co-factor, KRAB-associated protein-1 (KAP1), was demonstrated, and evidence supporting transcriptional repression by ZNFO was demonstrated using a GAL4-luciferase reporter system. Results of described studies demonstrate that ZNFO is a maternally-derived oocyte-specific nuclear factor required for early embryonic development in cattle, presumably functioning by repressing transcription. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect