Titanium dioxide nanoparticle exposure alters metabolic homeostasis in a cell culture model of the intestinal epithelium and Drosophila melanogaster
Autor: | Gretchen J. Mahler, Laura Palanker Musselman, Jonathan W. Richter, Anthony C. Fiumera, Gabriella Shull, Zhongyuan Guo, John H. Fountain |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Glucose uptake Biomedical Engineering Toxicology Article 03 medical and health sciences Lactobacillus rhamnosus In vivo Animals Homeostasis Humans Intestinal Mucosa Titanium biology Microvilli Chemistry Lacticaseibacillus rhamnosus Glucose transporter technology industry and agriculture Glucose analog biology.organism_classification Intestinal epithelium In vitro Cell biology 030104 developmental biology Drosophila melanogaster Glucose Caco-2 Nanoparticles Caco-2 Cells HT29 Cells |
DOI: | 10.6084/m9.figshare.6067616 |
Popis: | Nanosized titanium dioxide (TiO2) is a common additive in food and cosmetic products. The goal of this study was to investigate if TiO2 nanoparticles affect intestinal epithelial tissues, normal intestinal function, or metabolic homeostasis using in vitro and in vivo methods. An in vitro model of intestinal epithelial tissue was created by seeding co-cultures of Caco-2 and HT29-MTX cells on a Transwell permeable support. These experiments were repeated with monolayers that had been cultured with the beneficial commensal bacteria Lactobacillus rhamnosus GG (L. rhamnosus). Glucose uptake and transport in the presence of TiO2 nanoparticles was assessed using fluorescent glucose analog 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG). When the cell monolayers were exposed to physiologically relevant doses of TiO2, a statistically significant reduction in glucose transport was observed. These differences in glucose absorption were eliminated in the presence of beneficial bacteria. The decrease in glucose absorption was caused by damage to intestinal microvilli, which decreased the surface area available for absorption. Damage to microvilli was ameliorated in the presence of L. rhamnosus. Complimentary studies in Drosophila melanogaster showed that TiO2 ingestion resulted in decreased body size and glucose content. The results suggest that TiO2 nanoparticles alter glucose transport across the intestinal epithelium, and that TiO2 nanoparticle ingestion may have physiological consequences. |
Databáze: | OpenAIRE |
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