The value of immunohistochemical determination of topoisomerase IIα and Ki67 as markers of cell proliferation and malignant transformation in colonic mucosa
| Autor: | Jasminka Jakić-Razumović, Ivana Knezevic Stromar |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Adult
G2 Phase Male Epithelial dysplasia Pathology medicine.medical_specialty Histology Adolescent Pathology and Forensic Medicine Malignant transformation Antigens Neoplasm Biopsy medicine Humans Proliferation Marker Intestinal Mucosa Aged Retrospective Studies Aged 80 and over medicine.diagnostic_test business.industry Cell Differentiation Cell cycle Middle Aged medicine.disease Staining DNA-Binding Proteins Medical Laboratory Technology Cell Transformation Neoplastic DNA Topoisomerases Type II Ki-67 Antigen Dysplasia Colonic Neoplasms Immunohistochemistry topoisomerase IIa colon mucosa biopsy malignant alteration IBD dysplasia Female business Cell Division |
| Zdroj: | Applied immunohistochemistrymolecular morphology : AIMM. 22(7) |
| ISSN: | 1533-4058 |
| Popis: | Background: Considering the role of topoisomerase IIα in cell division, and its increased expression in the G2/M phase of the cell cycle, immunohistochemical staining for topoisomerase IIα might be used as a marker for cell proliferation or malignant transformation and compared with traditional proliferation marker (Ki67). Materials and methods: In this study, 215 consecutive patients, who had undergone colonoscopy with biopsy, were included. The biopsy samples were fixed in formaldehyde, embedded in paraffin, cut into 3- to 4-μm sections, stained with hematoxylin- eosin and analyzed under a light microscope for pathologic diagnosis. For determination of topoisomerase IIα and Ki67 expression, paraffin blocks were cut into 4- to 5-μm sections and immunohistochemically stained with antitopoisomerase IIα antibody and Ki67. The dysplasia level was determined in adenomas and inflammatory bowel disease (IBD) samples using standard histologic criteria, whereas the grade of differentiation was determined for all colonic carcinomas. Results: In 27 patients (12.6%) normal colonic mucosa was found, IBD was diagnosed in 72 patients (33.5%), 68 (31.6%) patients had adenomas, and 48 (22.3%) were diagnosed as colon carcinoma. In all samples with epithelial dysplasia, higher topoisomerase IIα expression was related to a higher level of dysplasia grade (P |
| Databáze: | OpenAIRE |
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