Increased binding and defective migration across fibronectin of cycling hematopoietic progenitor cells
| Autor: | Olivier Giet, Ivano Di Stefano, André Gothot, Roland Greimers, Dirk R. Van Bockstaele, Sandra Huygen, Yves Beguin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Integrin alpha4
Immunology Integrin Antigens CD34 Integrin alpha5 Biochemistry Cell Line Antigens CD Cell Adhesion Humans Bovine serum albumin Progenitor cell Interphase biology Cell Cycle Cell Biology Hematology Cell cycle Fetal Blood Hematopoietic Stem Cells Coculture Techniques Fibronectins Cell biology Fibronectin Chemotaxis Leukocyte Cell culture Cord blood biology.protein Human medicine Stem cell Protein Binding |
| Zdroj: | Blood |
| ISSN: | 0006-4971 |
| Popis: | Engraftment of hematopoietic progenitor cells has been shown to decrease during cell cycle transit. We studied cell cycle-associated changes in adhesion and migration of mitotically activated cord blood CD34(+) cells. Migration toward medium conditioned by the stromal-derived factor-1-producing cell line MS-5 was studied in bovine serum albumin- and fibronectin (Fn)-coated transwells. Migration was reduced in cycling CD34(+) cells and long-term culture-initiating cells (LTC-ICs) compared with their noncycling counterparts across Fn but not across bovine serum albumin. Conversely, Fn binding was higher in cycling CD34(+) cells and LTC-ICs compared with noncycling progenitor cells, while adhesion of both subsets to bovine serum albumin was undetectable. The contribution of alpha4 and alpha5 integrins in mediating adhesion and migration of activated CD34(+) cells onto Fn was analyzed by neutralization experiments. While alpha4-mediated Fn binding decreased during G(2)/M, alpha5 integrin-mediated adhesion increased during transit from G(0)/G(1) to S and G(2)/M phases. As for migration, the contribution of alpha4 integrin was similar in all phases, whereas alpha5-directed migration was lower in G(2)/M compared with G(0)/G(1) and S phases. Defective migration of cycling CD34(+) cells was not due to differences in alpha5 integrin expression. In conclusion, chemotaxis across Fn is less efficient in cycling progenitor cells in correlation with an increased Fn binding capacity. In addition, alpha4 and alpha5 integrin functions are independently modulated during cell cycle transit. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|