Evaluation of the Antibacterial Activity and Efflux Pump Reversal of Thymol and Carvacrol against Staphylococcus aureus and Their Toxicity in Drosophila melanogaster

Autor: Francisco Assis Bezerra da Cunha, Débora Feitosa Muniz, Valdir de Queiroz Balbino, Joycy Francely Sampaio dos Santos, Zildene de Sousa Silveira, Dárcio Luiz de Sousa Júnior, José Pinto de Siqueira Júnior, Henrique Douglas Melo Coutinho, Thiago Sampaio de Freitas, Nair Silva Macêdo, Ligia Cláudia Castro de Oliveira, Natália Martins, Cristina Rodrigues dos Santos Barbosa
Přispěvatelé: Instituto de Investigação e Inovação em Saúde
Rok vydání: 2020
Předmět:
Insecticides
Pharmaceutical Science
Pharmacology
medicine.disease_cause
Staphylococcus aureus / drug effects
Analytical Chemistry
chemistry.chemical_compound
terpenoids
Drosophila melanogaster / drug effects
Drug Discovery
Insecticides / pharmacology
Carvacrol
Thymol
0303 health sciences
Molecular Structure
Cymenes / pharmacology
Broth microdilution
bacterial resistance
Anti-Bacterial Agents / pharmacology
Anti-Bacterial Agents
Thymol / chemistry
Drosophila melanogaster
Chemistry (miscellaneous)
Staphylococcus aureus
Molecular Medicine
Efflux
Antibacterial activity
medicine.drug
Tetracycline
efflux pumps
carvacrol
Microbial Sensitivity Tests
Cymenes / chemistry
Article
lcsh:QD241-441
03 medical and health sciences
Minimum inhibitory concentration
lcsh:Organic chemistry
thymol
medicine
Animals
Physical and Theoretical Chemistry
030304 developmental biology
Dose-Response Relationship
Drug
030306 microbiology
Organic Chemistry
chemistry
Cymenes
Thymol / pharmacology
Zdroj: Molecules
Volume 25
Issue 9
Molecules, Vol 25, Iss 2103, p 2103 (2020)
ISSN: 1420-3049
DOI: 10.3390/molecules25092103
Popis: The antibacterial activity and efflux pump reversal of thymol and carvacrol were investigated against the Staphylococcus aureus IS-58 strain in this study, as well as their toxicity against Drosophila melanogaster. The minimum inhibitory concentration (MIC) was determined using the broth microdilution method, while efflux pump inhibition was assessed by reduction of the antibiotic and ethidium bromide (EtBr) MICs. D. melanogaster toxicity was tested using the fumigation method. Both thymol and carvacrol presented antibacterial activities with MICs of 72 and 256 µ
g/mL, respectively. The association between thymol and tetracycline demonstrated synergism, while the association between carvacrol and tetracycline presented antagonism. The compound and EtBr combinations did not differ from controls. Thymol and carvacrol toxicity against D. melanogaster were evidenced with EC50 values of 17.96 and 16.97 µ
g/mL, respectively, with 48 h of exposure. In conclusion, the compounds presented promising antibacterial activity against the tested strain, although no efficacy was observed in terms of efflux pump inhibition.
Databáze: OpenAIRE
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