Extracts of different pollen species and their effect on human tear fluid and an epithelial cell line
| Autor: | Otto Schmut, Jutta Horwath-Winter, Dieter Franz Rabensteiner, Jasmin Rabensteiner, Andrea Heidinger, Andreas Wedrich, Doris Lang-Loidolt, Gerold Schwantzer |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Male Cell Survival medicine.medical_treatment Toxicology medicine.disease_cause Cell Line Magnoliopsida 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Pollen medicine Humans Zymography Viability assay Polyacrylamide gel electrophoresis Aged Protease biology Chemistry food and beverages Epithelial Cells General Medicine Allergens Middle Aged biology.organism_classification Molecular biology In vitro Tears Oleaceae 030221 ophthalmology & optometry Female Adoxaceae |
| Zdroj: | Cutaneous and Ocular Toxicology. 38:93-103 |
| ISSN: | 1556-9535 1556-9527 |
| Popis: | Purpose Hazelnut and birch pollen are known to destroy tear film components and attack ocular surface cells. We investigated further pollen species from different plant families, whether they show similar effects on human tear fluid and an epithelial cell line in vitro, to provide a broad basis for further research on pollen reactions affecting the tear film and ocular surface. Materials and methods Regional pollen species from different plant families (Adoxaceae, Betulaceae, Fagaceae, Juglandaceae, Malvaceae, Oleaceae, Pinaceae, Plantaginaceae, Poaceae, Salicaceae, Sapindaceae) were collected. Their proteolytic activity was evaluated by Zymography. Human tear fluid and cells of an epithelial cell line were incubated with pollen extracts. Tear fluid was analyzed by Polyacrylamide gel electrophoresis (PAGE). Cytomorphology was assessed microscopically and cell viability by proliferation (MTS), water-soluble tetrazolium (WST-1) assay and the impedance-based xCELLigence real-time analysis (RTCA). Results Zymography revealed significant protease activity and PAGE showed the degradation of tear proteins by different pollen species. Cells incubated with pollen extracts presented dose- and time-dependent cytomorphological changes. MTS, WST-1, and RTCA revealed cytostatic as well as cytotoxic effects of pollen extracts. Conclusions Pollen species from different plant families exert proteolytic activity and degrade human tear fluid as well as epithelial cells, which may play a crucial role in the pathogenesis of allergic and non-allergic reactions affecting the ocular surface. |
| Databáze: | OpenAIRE |
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