The effect of tuberculin skin test and BCG vaccination on the expansion of PPD-specific IFN-gamma producing cells ex vivo
| Autor: | Martin O. C. Ota, Patrick K. Owiafe, Richard A. Adegbola, Helen McShane, Timothy Awine, Simon Donkor, Philip C. Hill, Hannah B. Ibanga, Roger H. Brookes |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Adult
Male Tuberculosis Adolescent Population Tuberculin chemical and pharmacologic phenomena complex mixtures Interferon-gamma Immune system Medicine Humans Lymphocytes education Tuberculosis Vaccines Cells Cultured education.field_of_study General Veterinary General Immunology and Microbiology business.industry Tuberculin Test Immunogenicity ELISPOT Public Health Environmental and Occupational Health medicine.disease Virology Mycobacterium bovis Vaccination Infectious Diseases Immunology Leukocytes Mononuclear Molecular Medicine Gambia business Ex vivo |
| Popis: | Understanding the immunogenicity of BCG in a population where it has failed will facilitate the design of new TB vaccines. We assessed the immunogenicity of M. bovis BCG over 12 months by ELISPOT assay. Forty-one adolescents and young Gambian male adults received a tuberculin skin test (TST) which was followed one week later by BCG vaccination, but the 23 control subjects received neither of these. TST alone significantly induced PPD-specific IFN-gamma producing cells. Twenty-three percent of subjects did not respond to BCG, which was associated with higher pre-existing ex vivo response to PPD. Paradoxically, amongst BCG responders there was a correlation between pre-existing response and subsequent response to BCG. We conclude that BCG is immunogenic, but this effector response is short-lived and can be limited in higher pre-existing anti-mycobacterial immunity, suggesting a possible threshold beyond which BCG immunogenicity is inhibited. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|