HPV genotype distribution among women with normal and abnormal cervical cytology presenting in a tertiary gynecology referral Clinic in Ethiopia
| Autor: | Shlomo Maayan, Yirgu Gebrehiwot, Solomon Gebressellassie, Bekure Tsegaye, Wondwossen Ergete, Orit Caplan, Dawit Wolday, Dana G. Wolf, Muluken Derese |
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| Rok vydání: | 2018 |
| Předmět: |
Cancer Research
medicine.medical_specialty HPV Referral Epidemiology HPV vaccines lcsh:RC254-282 lcsh:Infectious and parasitic diseases 03 medical and health sciences 0302 clinical medicine Cytology Genotype medicine Genotype distribution lcsh:RC109-216 030212 general & internal medicine Genotyping Gynecology Cervical cancer business.industry medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Sub-Sahara Africa Infectious Diseases Oncology 030220 oncology & carcinogenesis Tropical medicine Ethiopia business Nested polymerase chain reaction Research Article |
| Zdroj: | Infectious Agents and Cancer Infectious Agents and Cancer, Vol 13, Iss 1, Pp 1-8 (2018) |
| ISSN: | 1750-9378 |
| Popis: | Background Cervical cancer is the second most prevalent cancer among women of child-bearing age in Ethiopia. The aim of this study was to determine human papilloma virus (HPV) genotype distribution among HIV-negative women with normal and abnormal cervical cytology results. Methods We investigated a consecutive of 233 HIV-negative women between December 2008 and March 2009 presenting in a Tertiary Gynecology Referral Clinic in Ethiopia. Screening was done by Pap cytology and HPV detection and genotyping method was nested PCR (direct amplification with MY09/MY11 primers, followed by nested amplification with GP5/GP6 primers) and sequencing of the nested products. Sequencing of the non-purified nested PCR products was performed following re-amplification with Big dye terminator, using the GP6 primer. Results Of the 233 study participants, 92 (39.5%) had abnormal cytology. All women with abnormal cervical cytology had positive HPV DNA compared to only 48.9% of those presenting with normal cytology. Of these women, the frequency of high-risk (HR)-HPV was 83.2% and its prevalence in women with abnormal cervical cytology was significantly higher than those with normal cytology (92.4% vs. 71%, p |
| Databáze: | OpenAIRE |
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