Subcellular localization and expression of bamboo mosaic virus satellite RNA-encoded protein
| Autor: | Yau-Heiu Hsu, Chi-Ping Cheng, Morgan Chiu, Chuen-Chi Hsu, Ching-Chi Wang, Wei Chen, Choy-Chieng Lin, Chung-Mong Chen, Paramasivan Vijaya Palani, Na-Sheng Lin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
biology
Transcription Genetic Immunoelectron microscopy Bamboo mosaic virus RNA Nicotiana benthamiana Plant Bambusa oldhamii Viral Nonstructural Proteins biology.organism_classification Subcellular localization Virology Molecular biology Plant Leaves Open Reading Frames Viral Proteins Mosaic Viruses Helper virus otorhinolaryngologic diseases RNA Satellite RNA Viral Satellite (biology) Sasa |
| Zdroj: | The Journal of General Virology |
| ISSN: | 1465-2099 0022-1317 |
| Popis: | The satellite RNA of bamboo mosaic virus (satBaMV) has a single open reading frame encoding a non-structural protein, P20, which facilitates long-distance movement of satBaMV in BaMV and satBaMV co-infected plants. Immunohistochemistry and immunoelectron microscopy revealed that the P20 protein accumulated in the cytoplasm and nuclei in co-infected cells. P20 and the helper virus coat protein (CP) were highly similar in their subcellular localization, except that aggregates of BaMV virions were not labelled with anti-P20 serum. The BaMV CP protein was fairly abundant in mesophyll cells, whilst P20 was more frequently detected in mesophyll cells and vascular tissues. The expression kinetics of the P20 protein was similar to but slightly earlier than that of CP in co-infectedBambusa oldhamiiprotoplasts andNicotiana benthamianaleaves. However, satBaMV-encoded protein levels declined rapidly in the late phase of co-infection. During co-infection, in addition to the intact P20, a low-molecular-mass polypeptide of 16 kDa was identified as a P20 C-terminally truncated product; the possible method of generation of the truncated protein is discussed. |
| Databáze: | OpenAIRE |
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