Effects of a blend of garlic oil, nitrate and fumarate onin vitroruminal fermentation and microbial population
| Autor: | C. I. Mamvura, David Tinotenda Mbiriri, Nag-Jin Choi, Sangbuem Cho |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Rumen Population Allyl compound Garlic Oil Sulfides Nitric Acid 03 medical and health sciences chemistry.chemical_compound Fumarates Food Animals Nitrate Animals Food science Response surface methodology education education.field_of_study Bacteria Chemistry 0402 animal and dairy science food and beverages Fractional factorial design 04 agricultural and veterinary sciences 040201 dairy & animal science Allyl Compounds 030104 developmental biology Biochemistry Fermentation Animal Science and Zoology |
| Zdroj: | Journal of Animal Physiology and Animal Nutrition. 101:713-722 |
| ISSN: | 0931-2439 |
| Popis: | Although garlic oil and nitrate can effectively suppress ruminal methane (CH4 ) production in vitro, the application of these compounds is associated with suppressed total volatile fatty acid (VFA) concentration. On the other hand, the effectiveness of fumarate as a ruminal CH4 mitigating agent is variable but its application increases total VFA concentration. We therefore hypothesized that the different characteristics of the compounds can compensate for the shortcomings of the other. The objective of this study was to develop an optimal blend of garlic oil, nitrate and fumarate that can suppress in vitro ruminal CH4 without affecting total VFA concentration. Three ruminal in vitro fermentation experiments were carried out. The first one, a one factor at a time experiment was employed to investigate the effective concentration of each of the compounds on CH4 and VFA production by ruminal bacteria. We then applied the fractional factorial design and response surface methodology in the second experiment to determine optimal concentrations of the compounds in the blend. The optimal blending of garlic oil, fumarate and nitrate was determined to be 50 mg/l, 15 mm and 20 mm, respectively. This simulated optimal blend was verified in a 48 h in vitro batch fermentation experiment. The blend achieved the intended goal of suppressing CH4 whilst maintaining total VFA concentration. The blend and nitrate suppressed archaea populations (p |
| Databáze: | OpenAIRE |
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