Genotype identification and phylogenetic analysis of Enterocytozoon bieneusi in farmed black goats (Capra hircus) from China’s Hainan Province
| Autor: | Yan Zhang, Huan-Huan Zhou, Xin-Li Zheng, Tian-Ming Ma, Liu Quanwei, Feng Wang, Wei Zhao, Zhe Chao, Meng Qi, Sun Ruiping, Gang Lu, Wei Limin, Zong-Xi Cao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Veterinary medicine
China Farms Enterocytozoon bieneusi Genotype Veterinary (miscellaneous) 030231 tropical medicine lcsh:Infectious and parasitic diseases 03 medical and health sciences Feces 0302 clinical medicine fluids and secretions parasitic diseases DNA Ribosomal Spacer Microsporidiosis Capra hircus Prevalence Animals lcsh:RC109-216 Internal transcribed spacer DNA Fungal Close contact Phylogeny 030304 developmental biology 0303 health sciences Goat Diseases Minimal risk biology Phylogenetic tree Goats fungi ITS region virus diseases Genetic Variation Enterocytozoon biology.organism_classification Infectious Diseases Insect Science Animal Science and Zoology Parasitology Research Article |
| Zdroj: | Parasite Parasite, Vol 26, p 62 (2019) |
| ISSN: | 1776-1042 1252-607X |
| Popis: | Enterocytozoon bieneusi is an important pathogen commonly found in humans and animals. Farmed animals with close contact to humans are important hosts of E. bieneusi. The role of goats in the transmission of E. bieneusi, however, remains unclear. In this study, 341 fresh fecal samples of black goats were collected from five locations in Hainan Province, China. Enterocytozoon bieneusi was identified and genotyped by sequences of the internal transcribed spacer (ITS) region. Phylogenetic analysis was performed by constructing a neighbor-joining tree of the ITS gene sequences. The average prevalence of E. bieneusi in black goats was 24.0% (82/341) with rates ranging from 6.3% (4/63) to 37.2% (32/86) across the locations (χ2 = 17.252, p E. bieneusi were identified, including six known genotypes: CHG5 (n = 47); CHG3 (n = 23); CHG2 (n = 4); CM21 (n = 3); D (n = 2); and AHG1 (n = 1), and two novel genotypes termed HNG-I (n = 1) and HNG-II (n = 1). In the phylogenetic tree, genotype D was clustered into Group 1 and the other identified genotypes were included in Group 2. This represents the first report identifying E. bieneusi in black goats from Hainan Province, with a high prevalence and wide occurrence demonstrated. The two new genotypes identified provide additional insights into the genotypic variations in E. bieneusi. Due to the small percentage of zoonotic genotypes in these animals, there is minimal risk of zoonotic transmission of E. bieneusi. |
| Databáze: | OpenAIRE |
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