Asymmetric histone inheritance via strand-specific incorporation and biased replication fork movement
| Autor: | Joseph G. Gall, Rajesh Ranjan, Jonathan Snedeker, Zehra F. Nizami, Matthew Wooten, Jie Xiao, Xinxing Yang, Elizabeth Urban, Xin Chen, Jee Min Kim |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
DNA Replication
Male Cell division Chromatids Article Epigenesis Genetic Histone H4 Histones 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Structural Biology Testis Animals Drosophila Proteins Epigenetics Transgenes Molecular Biology 030304 developmental biology Chromatin Fiber 0303 health sciences biology Adult Germline Stem Cells Chemistry Asymmetric Cell Division DNA replication Chromatin Cell biology Histone Drosophila melanogaster Gene Expression Regulation biology.protein 030217 neurology & neurosurgery DNA |
| Zdroj: | Nature structural & molecular biology |
| ISSN: | 1545-9985 |
| Popis: | SUMMARY Many stem cells undergo asymmetric division to produce a self-renewing stem cell and a differentiating daughter cell. Here we show that, similarly to H3, histone H4 is inherited asymmetrically in Drosophila melanogaster male germline stem cells undergoing asymmetric division. In contrast, both H2A and H2B are inherited symmetrically. By combining superresolution microscopy and chromatin fiber analyses with proximity ligation assays on intact nuclei, we find that old H3 is preferentially incorporated by the leading strand whereas newly synthesized H3 is enriched on the lagging strand. Using a sequential nucleoside analog incorporation assay, we detect a high incidence of unidirectional replication fork movement in testes-derived chromatin and DNA fibers. Biased fork movement coupled with a strand preference in histone incorporation would explain how asymmetric old and new H3 and H4 are established during replication. These results suggest a role for DNA replication in patterning epigenetic information in asymmetrically dividing cells in multicellular organisms. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink