Development of a Multiplex PCR Assay for Detection and Discrimination of Pathogenic and Saprophytic Leptospira in Water
| Autor: | Rathinsabapthi Pasupathi, Gayathri Rethinavelu, Mohandass Ramya, Archana Vishwakarma |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
biology 030231 tropical medicine 030106 microbiology water multiplex pcr waterborne infection biology.organism_classification Applied Microbiology and Biotechnology Microbiology QR1-502 03 medical and health sciences 0302 clinical medicine Leptospira Multiplex polymerase chain reaction leptospirosis pathogenic serovars Biotechnology |
| Zdroj: | Journal of Pure and Applied Microbiology, Vol 15, Iss 1, Pp 240-245 (2021) |
| ISSN: | 0973-7510 |
| Popis: | Leptospirosis is a zoonosis prevalent in tropical countries and affects animals and humans alike. Leptospira interrogans, the causative organism for this waterborne infection, spreads through the urine of infected animals. There is a direct link between contaminated water and Leptospira outbreaks. This study reports a rapid assay to detect and differentiate pathogenic Leptospira from non-pathogenic in environmental water using multiplex PCR. The assay uses primers targeting the Lipl32 and Lipl21 gene. The multiplex PCR has been standardized using 11 pathogenic and one saprophytic serovar of Leptospira. The analytical sensitivity of the developed method was evaluated with different concentrations of template DNA. This method was used to screen water samples collected from 20 different sources from Chengalpattu town in Kancheepuram District, Tamil Nadu, India. Of the 20 water samples screened, 13 samples tested positive for pathogenic Leptospira, and seven samples tested negative. Four water samples were found to carry both pathogenic and saprophytic species. The developed multiplex PCR assay is highly useful for detecting and distinguishing pathogenic and saprophytic leptospires in water. |
| Databáze: | OpenAIRE |
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