Novel gene-modified-tissue engineering of cartilage using stable transforming growth factor-β1-transfected mesenchymal stem cells grown on chitosan scaffolds
| Autor: | Chang-An Guo, Xue-Guang Liu, Jian-Zhong Huo, Zheng-Rong Chen, Xue-Jun Wen, Chun Jiang |
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| Rok vydání: | 2007 |
| Předmět: |
Cartilage
Articular Male Mesenchyme Bioengineering Biology Mesenchymal Stem Cell Transplantation Transfection Applied Microbiology and Biotechnology Transforming Growth Factor beta1 Extracellular matrix Tissue engineering medicine Animals Aggrecans RNA Messenger Collagen Type II DNA Primers Chitosan Drug Carriers Base Sequence Tissue Engineering Cartilage Mesenchymal stem cell Mesenchymal Stem Cells Lipids Matrix Metalloproteinases Fibronectins Cell biology medicine.anatomical_structure Lipofectamine Liposomes Immunology Female Rabbits Stem cell Biotechnology |
| Zdroj: | Journal of Bioscience and Bioengineering. 103:547-556 |
| ISSN: | 1389-1723 |
| DOI: | 10.1263/jbb.103.547 |
| Popis: | Rabbit bone marrow-derived mesenchymal stem cells (MSCs) were stably transfected with the TGF-beta1 gene in monolayer culture using Lipofectamine 2000. After transfection, the expression of cartilage-specific extracellular matrix was upregulated, whereas matrix metalloproteinases 1 and 3 (MMP 1 and 3) protein expressions and enzymatic activities were downregulated. Autologous MSCs modified with the TGF-beta1 gene were seeded into chitosan scaffolds to construct gene-modified cartilage, which was then implanted into the full-thickness articular cartilage defects of rabbits' knees. Twelve weeks after implantation, the defects were filled with regenerated hyaline-like cartilage tissue as confirmed by the positive immunohistochemical staining of collagen type II and intense toluidine blue staining of proteoglycan. Our findings suggest that the repair of cartilage defects can be enhanced by TGF-beta1 gene-modified-tissue engineering of cartilage on the basis of a strategy using MSCs, chitosan, and liposomal transfection. |
| Databáze: | OpenAIRE |
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