A Quantitative Method for Measuring Antipsoriatic Activity of Drugs by the Mouse Tail Test
| Autor: | Brigitte Bosman, Volker Hess, Elmar Friderichs, Theo Matthiesen |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
Tail Evaluation system Physiology Stereochemistry Drug Evaluation Preclinical Mice Inbred Strains Tretinoin Topical treatment Dermatology Pharmacology Mice Animal model Dithranol medicine Animals Psoriasis Chemistry General Medicine Anthralin Orthokeratosis Tars Investigation methods Mechanism of action medicine.symptom medicine.drug |
| Zdroj: | Skin Pharmacology and Physiology. 5:41-48 |
| ISSN: | 1660-5535 1660-5527 |
| DOI: | 10.1159/000211016 |
| Popis: | Topical treatment of the mouse tail with antipsoriatic drugs enhances orthokeratotic cell differentiation in the epidermal scales. We developed a new evaluation system for this test which allows quantification of drug efficacy. Drugs were applied topically, once daily, 5 times a week, for 2 weeks. Two hours after the last treatment the animals were sacrificed, longitudinal sections of the tail skin were made and prepared for histological examination (hematoxylineosin staining). As indicator of orthokeratosis (OK), the length of the granular layer per scale was measured microscopically with a semiautomatic image evaluation unit and related to the total scale length(= % OK per scale). Drug activity was defined by the increase in the total length of orthokeratotic regions, 100% activity corresponds to a granular layer extending over the whole scale length. In this model dithranol and retinoic acid dose-dependently increased orthokeratosis up to 75 and 79%, respectively. Beech tar, in a concentration of 5 % induced a 19 % increase in orthokeratosis. Morphometric quantification by image analysis of the conversion of parakeratotic into orthokeratotic regions in mouse tail scales induced by topical drug treatment seems to be a suitable and reliable procedure to investigate new drugs from which antipsoriatic acitivity may be expected. |
| Databáze: | OpenAIRE |
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