Evaluation of Mollicutes microorganisms in respiratory disease of cattle and their relationship to clinical signs
| Autor: | L. Miranda Marques, B.L. Mendonça Ribeiro, Gabriela Tortorelli, Jorge Timenetsky, Lilian Gregory, N. Carrillo Gaeta |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
040301 veterinary sciences Dispar Cattle Diseases Bovine respiratory disease Buiatrics Standard Article MYCOPLASMA medicine.disease_cause Diseases of cattle Polymerase Chain Reaction Microbiology 0403 veterinary science 03 medical and health sciences Mycoplasma medicine Animals Mycoplasma Infections Respiratory Tract Infections Dairy cattle FOOD AND FIBER ANIMAL Tenericutes General Veterinary Respiratory tract infections biology Bovine 04 agricultural and veterinary sciences biology.organism_classification medicine.disease Standard Articles 030104 developmental biology Case-Control Studies Respiratory Mollicutes Cattle Gram-Negative Bacterial Infections |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Journal of Veterinary Internal Medicine |
| Popis: | Background Bovine respiratory disease (BRD) is an important problem in cattle production that is responsible for economic losses in dairy herds. Mycoplasma spp. are described as an important etiological agent of BRD. Hypothesis To evaluate the occurrence of the most important mycoplasmas in the lower respiratory tract of healthy and BRD cattle in relationship to clinical signs of BRD. Animals Sixty young dairy cattle were classified as healthy (n = 32) or cattle showing clinical signs of BRD (n = 28). Methods Tracheal lavage samples were collected and added to tubes containing Hayflick media. Mycoplasma spp. were identified by the presence of “fried egg” like colonies, biochemical tests and polymerase chain reaction (PCR). Occurrence of Mollicutes, M. bovis, M. mycoides subsp. mycoides SC and M. dispar was evaluated. The association between clinical signs of BRD and the presence of Mycoplasma spp. also was evaluated. Results Colonies were obtained from a 1-year-old BRD calf only. However, species identification was not possible. Mollicutes (P = .035) and M. dispar (P = .036) were more common in BRD cattle. The relationship between Mollicutes and crackle (P = .057) was not significant. M. dispar was associated to tachypnea (P = .045) and mixed dyspnea (P = .003). Relationships to heart rate (P = .062) and crackle (P = .062) were not significant. Conclusions and clinical importance The results confirmed the importance of mycoplasma as an etiologic agent of BRD and suggested M. dispar as part of the respiratory microbiota and its possible role in the development of BRD. |
| Databáze: | OpenAIRE |
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