Concerning the mechanism of increased thermogenesis in rats treated with dehydroepiandrosterone

Autor: Valentina Bobyleva, Monica Bellei, Nancy Kneer, Henry A. Lardy, Daniela Battelli
Rok vydání: 1993
Předmět:
Male
medicine.medical_specialty
Physiology
Body Temperature Regulation Body Weight Dehydroepiandrosterone/*pharmacology Energy Metabolism FAD/metabolism Glycerophosphates/metabolism Liver/cytology/metabolism Mitochondria
Liver/drug effects/metabolism NADP/metabolism
Malic enzyme
Dehydrogenase
Glycerolphosphate Dehydrogenase
Mitochondria
Liver
Biology
Mitochondrion
Aconitase
Models
Biological
Citric Acid
Oxidative Phosphorylation
Rats
Sprague-Dawley
chemistry.chemical_compound
Cytosol
Malate Dehydrogenase
Internal medicine
medicine
Animals
Citrates
Cells
Cultured
Dihydroxyacetone phosphate
Body Weight
Cell Biology
Dehydroepiandrosterone
Rats
Endocrinology
Glycerol-3-phosphate dehydrogenase
Isocitrate dehydrogenase
Biochemistry
chemistry
Liver
Dihydroxyacetone Phosphate
Glycerophosphates
Flavin-Adenine Dinucleotide
NAD+ kinase
Energy Metabolism
Glycolysis
NADP
Body Temperature Regulation
Zdroj: Journal of bioenergetics and biomembranes. 25(3)
ISSN: 0145-479X
Popis: Dehydroepiandrosterone (DHEA) treatment of rats decreases gain of body weight without affecting food intake; simultaneously, the activities of liver malic enzyme and cytosolic glycerol-3-P dehydrogenase are increased. In the present study experiments were conducted to test the possibility that DHEA enhances thermogenesis and decreases metabolic efficiency via trans-hydrogenation of cytosolic NADPH into mitochondrial FADH2 with a consequent loss of energy as heat. The following results provide evidence which supports the proposed hypothesis: (a) the activities of cytosolic enzymes involved in NADPH production (malic enzyme, cytosolic isocitrate dehydrogenase, and aconitase) are increased after DHEA treatment; (b) cytosolic glycerol-3-P dehydrogenase may use both NAD+ and NADP+ as coenzymes; (c) activities of both cytosolic and mitochondrial forms of glycerol-3-P dehydrogenase are increased by DHEA treatment; (d) cytosol obtained from DHEA-treated rats synthesizes more glycerol-3-P during incubation with fructose-1,6-P2 (used as source of dihydroxyacetone phosphate) and NADP+; the addition of citratein vitro further increases this difference; (e) mitochondria prepared from DHEA-treated rats more rapidly consume glycerol-3-P added exogenously or formed endogenously in the cytosol in the presence of fructose-1,6-P2 and NADP+.
Databáze: OpenAIRE
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