A Papaver somniferum 10-gene cluster for synthesis of the anticancer alkaloid noscapine
| Autor: | Valeria Gazda, Thilo Winzer, Yi Li, Ian A. Graham, Roxana Teodor, Fergus Meade, Fabián E. Vaistij, Tim Bowser, Filip Kaminski, Carol Walker, Tony R. Larson, Zhesi He, Marcelo Kern |
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| Rok vydání: | 2012 |
| Předmět: |
Noscapine
Bacterial artificial chromosome education.field_of_study Multidisciplinary Stereochemistry Population Molecular Sequence Data Opium Poppy Biology biology.organism_classification Genes Plant Antineoplastic Agents Phytogenic Biochemistry Papaver Multigene Family Gene cluster medicine Gene silencing education Gene medicine.drug |
| Zdroj: | Science (New York, N.Y.). 336(6089) |
| ISSN: | 1095-9203 |
| Popis: | Alkaloid Synthetic Pathway Noscapine, a nonaddictive alkaloid found in the opium poppy, can be used as a cough suppressant and a tubulin-binding antitumor agent. Winzer et al. (p. 1704 , published online 31 May; see the Perspective by DellaPenna and O'Connor ) found that a cluster of 10 genes were key to the production of noscapine. Poppies homozygous for this gene cluster produced high levels of noscapine, heterozygous poppies produced low levels of noscapine, and those poppies lacking the gene cluster produced no noscapine. Silencing individual genes in turn and analyzing the accumulation of intermediate metabolites allowed the biosynthetic pathway of noscapine to be elucidated. |
| Databáze: | OpenAIRE |
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