Poly(amidoamine) dendrimer-coated magnetic nanoparticles for the fast purification and selective enrichment of glycopeptides and glycans
| Autor: | Bekir Salih, Ali Serol Ertürk, Hacı Mehmet Kayili, Gökhan Elmacı |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
Glycan Chromatography Glycosylation biology 010401 analytical chemistry Amidoamine Filtration and Separation Poly(amidoamine) 010402 general chemistry 01 natural sciences Glycopeptide 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound chemistry Dendrimer biology.protein Bovine serum albumin Glycoprotein |
| Zdroj: | Journal of separation scienceREFERENCES. |
| ISSN: | 1615-9314 |
| Popis: | Glycosylated proteins modulate various important functions of organisms. To reveal the functions of glycoproteins, in-depth characterization studies are necessary. Although mass spectrometry is a very efficient tool for glycoproteomic and glycomic studies, efficient sample preparation methods are required prior to analyses. In the study, poly(amidoamine) dendrimer-coated magnetic nanoparticles were presented for the specific enrichment and fast purification of glycopeptides and glycans. The enrichment and purification performance of the developed method was evaluated both at the glycopeptide, and the glycan level using several standard glycoprotein digests and released glycan samples. The poly(amidoamine) dendrimer-coated magnetic nanoparticles not only showed selective affinity (Immunoglobulin G/Bovine Serum Albumin, 1/10 by weight) to glycopeptides and released glycans but also good sensitivity (0.4 ng/µL for Immunoglobulin G) for glycoproteomic and glycomic applications. Thirty-five glycopeptides of Immunoglobulin G were detected after enrichment with poly(amidoamine) dendrimer-coated magnetic nanoparticles. In addition, 55 18 O tagged deamidated glycopeptides belonging to human plasma glycoproteome were confirmed. Finally, fifty 2-aminobenzoic acid, and 30 procainamide-labelled human plasma N-glycans released from human plasma glycoproteins were determined after purifications. The results indicate that the proposed enrichment and purification method using poly(amidoamine) dendrimer-coated magnetic nanoparticles could be simply adjusted to sample preparation methods. |
| Databáze: | OpenAIRE |
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