Distinct skeletal muscle fiber characteristics and gene expression in diet-sensitive versus diet-resistant obesity
| Autor: | Sujoy Ghosh, Benjamin Hill, Ruth McPherson, Erin L. Seifert, Brittany Beauchamp, Shelby A. Gorman, Nihan Kavaslar, Martin F. Gerrits, Joan S. Stuart, Anastasia Tour, Robert Dent, Mary-Ellen Harper |
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| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Muscle Fibers Skeletal oxidative phosphorylation QD415-436 Carbohydrate metabolism Biology Biochemistry Quadriceps Muscle Muscle hypertrophy chemistry.chemical_compound Endocrinology Weight loss Internal medicine Weight Loss medicine Humans Obesity Phosphorylation Genome Fatty acid metabolism Gene Expression Profiling Fatty Acids Skeletal muscle thermogenesis Hypertrophy Organ Size Cell Biology Middle Aged medicine.disease fiber type Diet Mitochondria Up-Regulation Glucose Phenotype medicine.anatomical_structure chemistry Organ Specificity Female medicine.symptom Patient-Oriented and Epidemiological Research Oxidation-Reduction Thermogenesis Weight gain |
| Zdroj: | Journal of Lipid Research, Vol 51, Iss 8, Pp 2394-2404 (2010) |
| ISSN: | 0022-2275 |
| DOI: | 10.1194/jlr.p005298 |
| Popis: | Inter-individual variability in weight gain and loss under energy surfeit and deficit conditions, respectively, are well recognized but poorly understood phenomena. We documented weight loss variability in an intensively supervised clinical weight loss program and assessed skeletal muscle gene expression and phenotypic characteristics related to variable response to a 900 kcal regimen. Matched pairs of healthy, diet-compliant, obese diet-sensitive (ODS) and diet-resistant (ODR) subjects were defined as those in the highest and lowest quintiles for weight loss rate. Physical activity energy expenditure was minimal and comparable. Following program completion and weight stabilization, skeletal muscle biopsies were obtained. Gene expression analysis of rectus femoris and vastus lateralis indicated upregulation of genes and gene sets involved in oxidative phosphorylation and glucose and fatty acid metabolism in ODS compared with ODR. In vastus lateralis, there was a higher proportion of oxidative (type I) fibers in ODS compared with ODR women and lean controls, fiber hypertrophy in ODS compared with ODR women and lean controls, and lower succinate dehydrogenase in oxidative and oxidative-glycolytic fibers in all obese compared with lean subjects. Intramuscular lipid content was generally higher in obese versus lean, and specifically higher in ODS vs. lean women. Altogether, our findings demonstrate differences in muscle gene expression and fiber composition related to clinical weight loss success. |
| Databáze: | OpenAIRE |
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